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When isolating cells using EasySep™ or RoboSep™ cell separation systems, the traditionally recommended buffer is 2% FBS in Phosphate Buffered Saline (PBS, Ca²⁺ and Mg²⁺ free), with 1 mM EDTA. For your convenience, you can purchase this buffer ready-made from ���ϳԹ� (EasySep™ Buffer, 1 L, Catalog #20144 or RoboSep™ Buffer, 250 mL, Catalog #20104).

However, if you prefer to avoid the use of serum, you could use an alternative buffer consisting of PBS (Ca²⁺ and Mg²⁺free), supplemented with 0.5% BSA or HSA, and 2 mM EDTA. We provide a serum-free buffer which contains 0.5% BSA (RoboSep Buffer 2, 250 mL, Catalog #20164). If required, you could also substitute the PBS for Hank's Balanced Salt Solution (HBSS, Ca²⁺ and Mg²⁺ free, Catalog #37250).

We cannot recommend using other media (such as RPMI, DMEM/F12 or DMEM) as the buffer for cell separations, as we have not tested this. Our cell separation kits have only been validated using the recommended buffers above. More importantly, most media (e.g. DMEM) is not buffered, unless it's maintained in an environment with 5-10% CO₂. Outside of a CO₂ incubator, the pH of the media will likely change, which is not optimal.

For further assistance and information, please contact techsupport@stemcell.com.

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