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(A) The biological activity of Human Recombinant LIF was tested by its ability to promote the proliferation of TF-1 cells. Cell proliferation
was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell
proliferation is at 50% of maximum. The EC50 for this effect is 0.09 ng/mL.
(B) 2 μg of Human Recombinant LIF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by
Coomassie Blue staining. Human Recombinant LIF has a predicted molecular mass of 19.7 kDa.
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ºÚÁÏ³Ô¹Ï TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.
