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Anti-Human CD235ab (Glycophorin A/B) Antibody, Clone HIR2

Mouse monoclonal IgG2b antibody against human CD235ab (glycophorin A/B)

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Anti-Human CD235ab (Glycophorin A/B) Antibody, Clone HIR2

Mouse monoclonal IgG2b antibody against human CD235ab (glycophorin A/B)

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Mouse monoclonal IgG2b antibody against human CD235ab (glycophorin A/B)
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Overview

The HIR2 (GA-R2) antibody recognizes an epitope common to the N-terminal region of human CD235a (glycophorin A) and CD235b (glycophorin B), homologous type I sialoglycoproteins of the erythrocyte (red blood cell) membrane that bear the antigenic determinants for the MN and Ss blood groups. CD235ab is expressed on early- and late-stage erythroblasts, mature erythrocytes, and erythroid cell lines such as K562 and HEL, but not on other cell types. CD235a is abundantly expressed whereas CD235b is a relatively minor membrane component. The proteins are believed to provide a large mucin-like surface to erythrocytes that acts to minimize aggregation in the circulation. The HIR2 antibody binds with higher affinity to CD235a than CD235b, and agglutinates untreated erythrocytes.
Subtype
Primary Antibodies
Target Antigen
CD235ab (Glycophorin A/B)
Alternative Names
CD235a, CD235ab, CD235b, Glycophorin A, Glycophorin B, GPA, GPB, GYPA, GYPB, MN sialoglycoprotein, PAS-2, PAS-3, Sialoglycoprotein alpha, Sialoglycoprotein delta, SS-active sialoglycoprotein
Reactive Species
Human
Conjugation
APC, Biotin, FITC, PE, Unconjugated
Host Species
Mouse
Cell Type
Red Blood Cells
Species
Human
Application
CyTOF, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting
Area of Interest
Immunology
Clone
HIR2 (GA-R2)
Gene ID
2993/2994
Isotype
IgG2b, kappa

Data Figures

Flow Cytometry Analysis of Human Whole Blood

Figure 1. Data for Unconjugated

Flow cytometry analysis of human whole blood labeled with Anti-Human CD235ab (Glycophorin A/B) Antibody, Clone HIR2, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI) (filled histogram), or Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11 (Catalog #60072), followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (solid line histogram).

Data for APC-Conjugated

Figure 2. Data for APC-Conjugated

Flow cytometry analysis of human whole blood labeled with Anti-Human CD235ab (Glycophorin A/B) Antibody, Clone HIR2, APC (filled histogram) or Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, APC (Catalog #60072AZ, solid line histogram).

Data for Biotin-Conjugated

Figure 3. Data for Biotin-Conjugated

Flow cytometry analysis of human whole blood labeled with Anti-Human CD235ab (Glycophorin A/B) Antibody, Clone HIR2, Biotin followed by streptavidin (SAV) APC (filled histogram), or Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, Biotin (Catalog #60072BT) followed by SAV APC (solid line histogram).

Data for FITC-Conjugated

Figure 4. Data for FITC-Conjugated

Flow cytometry analysis of human whole blood labeled with Anti-Human CD235ab (Glycophorin A/B) Antibody, Clone HIR2, FITC (filled histogram) or Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, FITC (Catalog #60072FI, solid line histogram).

Data for PE-Conjugated

Figure 5. Data for PE-Conjugated

Flow cytometry analysis of human whole blood labeled with Anti-Human CD235ab (Glycophorin A/B) Antibody, Clone HIR2, PE (filled histogram) or Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, PE (Catalog #60072PE, solid line histogram).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Name
Catalog #
60111BT
Lot #
All
Language
English
Document Type
Product Name
Catalog #
60111, 60111.1
Lot #
All
Language
English
Document Type
Product Name
Catalog #
60111PE, 60111PE.1
Lot #
All
Language
English
Document Type
Product Name
Catalog #
60111AZ, 60111AZ.1
Lot #
All
Language
English
Document Type
Product Name
Catalog #
60111FI.1, 60111FI
Lot #
All
Language
English
Document Type
Product Name
Catalog #
60111BT
Lot #
All
Language
English
Document Type
Product Name
Catalog #
60111, 60111.1
Lot #
All
Language
English
Document Type
Product Name
Catalog #
60111PE, 60111PE.1
Lot #
All
Language
English
Document Type
Product Name
Catalog #
60111AZ, 60111AZ.1
Lot #
All
Language
English
Document Type
Product Name
Catalog #
60111FI.1, 60111FI
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (2)

Brochure
Human Immune Cytokines
Wallchart
Human Immune Cytokines

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