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The HI100 antibody reacts with an extracellular epitope on the CD45RA isoform of CD45, a type I transmembrane glycoprotein and member of the protein tyrosine phosphatase family (receptor class 1/6 subfamily). Alternative splicing of exons 4, 5, and 6 that encode the extracellular RA, RB, and RC polypeptides of CD45 gives rise to several isoforms with molecular masses of 180 - 240 kDa. Excision of exon 4 generates the ~220-kDa CD45RA isoform, which is expressed on naïve T cells, subsets of B cells and monocytes, and medullary thymocytes. CD45RA functions to enhance signaling through both the T cell receptor and B cell receptor. The HI100 antibody is commonly used in combination with antibodies against the CD45RO isoform to discern naïve and memory T cells and is useful for identifying the suppressor/inducer subset of CD4+ lymphocytes. The proportion of CD45RA+ T cells typically decreases with age.
Subtype
Primary Antibodies
Target Antigen
CD45RA
Reactive Species
Chimpanzee, Human
Conjugation
APC-Cyanine7, Biotin, PE, Unconjugated
Host Species
Mouse
Cell Type
Granulocytes and Subsets, Hematopoietic Stem and Progenitor Cells, Leukocytes, Lymphocytes
Figure 1. Data for Anti-Human CD45RA Antibody, Clone HI100, APC-Cy7
Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs; gated on viable lymphocytes) (A) labeled with Anti-Human CD45RA Antibody, Clone HI100, APC-Cyanine7 and anti-human CD45RO antibody, clone UCHL1, APC, (B) labeled with a mouse IgG2b, kappa isotype control antibody, clone MPC-11, APC-Cyanine7 and anti-human CD45RO antibody, clone UCHL1, APC, and (C) labeled with Anti-Human CD45RA Antibody, Clone HI100, APC-Cyanine7 (filled histogram) or a mouse IgG2b, kappa APC-Cyanine7 isotype control antibody (solid line histogram).
Figure 2. Data for Anti-Human CD45RA Antibody, Clone HI100, Biotin
Flow cytometry analysis of human PBMCs (A) labeled with Anti-Human CD45RA Antibody, Clone HI100, Biotin, followed by streptavidin (SAV) PE, and anti-human CD45RO antibody, clone UCHL1, APC. (B) Flow cytometry analysis of human PBMCs labeled with a mouse IgG2b, kappa Biotin isotype control antibody followed by SAV PE, and anti-human CD45RO antibody, clone UCHL1, APC. (C) Flow cytometry analysis of human PBMCs labeled with Anti-Human CD45RA Antibody, Clone HI100, Biotin, followed by SAV PE (filled histogram) or a mouse IgG2b, kappa Biotin isotype control antibody, followed by SAV PE (solid line histogram). Viable lymphocytes were gated for analysis.
Figure 3. Data for Anti-Human CD45RA Antibody, Clone HI100, PE
Flow cytometry analysis of human PBMCs (gated on viable lymphocytes) (A) labeled with Anti-Human CD45RA Antibody, Clone HI100, PE, and anti-human CD45RO antibody, clone UCHL1, APC, (B) labeled with a mouse IgG2b, kappa PE isotype control antibody and anti-human CD45RO antibody, clone UCHL1, APC, and (C) labeled with Anti-Human CD45RA Antibody, Clone HI100, PE (filled histogram) or a mouse IgG2b, kappa PE isotype control antibody (solid line histogram).
Figure 4. Data for Anti-Human CD45RA Antibody, Clone HI100, Unconjugated
Flow cytometry analysis of human PBMCs (gated on viable lymphocytes) (A) labeled with Anti-Human CD45RA Antibody, Clone HI100, followed by a goat anti-mouse IgG2b, PE, and anti-human CD45RO antibody, clone UCHL1, APC, (B) labeled with a mouse IgG2b, kappa isotype control antibody, Clone MPC-11, followed by a goat anti-mouse IgG2b, PE, and anti-human CD45RO antibody, clone UCHL1, APC,and (C) labeled with Anti-Human CD45RA Antibody, Clone HI100, followed by a goat anti-mouse IgG2b, PE (filled histogram) or a mouse IgG2b, kappa isotype control antibody followed by a goat anti-mouse IgG2b, PE (solid line histogram).
This product is designed for use in the following research area(s) as part
of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we
offer to support each research area.
Mouse monoclonal IgG2a antibody against human, chimpanzee, common marmoset CD45RO
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Anti-Human CD45RA, Clone HI100, Biotin
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT ϳԹ, REFER TO WWW.ϳԹ.COM/COMPLIANCE.
