ʴǰ™
ʴǰ™ Transfection System
Gentle Intracellular Delivery with High Efficiency and Viability
Intracellular delivery of molecules is crucial for advancing biological research and developing innovative cell and gene-based therapies. Traditional methods for transfecting cells are often limited in their ability to deliver a wide range of cargoes across diverse cell types. Additionally, these methods can inadvertently alter gene expression and disrupt cell function.
To address your cell engineering needs, we developed the ʴǰ™ Transfection System—an instrument offering versatility in delivering a variety of macromolecules into the cytosol of cells. Using gentle microfluidic technology, the system provides a simple, intuitive workflow that can be tailored to your specific research applications.
How Does ʴǰ™ Work?
Consisting of a benchtop instrument, the system uses pressure to squeeze the cells at high speeds through parallel microfluidic channels embedded in a single-use delivery cartridge (included in the ʴǰ™ Transfection Kits). This creates transient cell membrane pores, a process known as mechanoporation, which enables the entry of target cargoes into the cytosol before the membrane reseals.
Why Use ʴǰ™?
- Achieve high delivery and viability for your cells
- Fine-tune a single parameter to identify optimal delivery and viability conditions
- Deliver cargoes intracellularly without altering cell quality
- Transfect up to 10 million cells per second
- Deliver a wide variety of cargoes to a broad range of cell types
Figure 1. ʴǰ™ Transfection System Workflow
This figure illustrates the ʴǰ™ Transfection System workflow in three straightforward steps: 1) prepare cells sourced from freshly isolated or frozen samples using cell isolation technology such as ⳧™; 2) deliver one or more desired cargoes into your cells using the ʴǰ™ Transfection System; 3) analyze the delivery efficiency for downstream applications.
ʴǰ™ Products
ʴǰ™ Transfection System
Compatible Cell Types
Compatibility defined by the kit
Compatible Target Cargoes
Compatibility defined by the kit
Size
- 40.6 cm W x 31.7 cm D x 20.3 cm H (16" W x 12.5” D x 8” H)
- 13 kg (29 lb) without gas tank
ʴǰ™ Transfection Kit 300
Compatible Cell Types
Human cells
• Unactivated Pan T cells
• Unactivated CD4 T cells
• Unactivated CD8 T cells
• Unactivated Regulatory T cells
• Unactivated Natural killer (NK) cells
• CD34+ hematopoietic stem and progenitor cells (HSPCs)
• Peripheral blood mononuclear cells (PBMCs)
Compatible Target Cargoes
Small Molecules
Polymers
Peptides
Proteins, Antibodies
Small RNA (e.g. miRNA)
Large RNA (e.g. mRNA)
CRISPR RNP
Size
12 reactions
Collapse Details
ʴǰ™ Transfection System
Compatible Cell Types
Compatible Target Cargoes
Size
- 40.6 cm W x 31.7 cm D x 20.3 cm H (16" W x 12.5” D x 8” H)
- 13 kg (29 lb) without gas tank
ʴǰ™ Transfection Kit 300
Compatible Cell Types
Compatible Target Cargoes
Size
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The purchase of a ʴǰ™ Transfection System, ʴǰ™ Delivery Cartridges, and associated reagents (“ʴǰ™ Products”) conveys to the purchaser a limited, non-exclusive, non-transferable license to use ʴǰ™ products, in accordance with ϳԹ’s Terms and Conditions of Sale (www.stemcell.com/terms-and-conditions-general) and all applicable laws and regulations, and solely for research-use-only applications (which applications exclude any commercial application or any therapeutic, prophylactic, diagnostic application, as well as any development and/or commercialization of products therewith or developed therefrom). ϳԹ or its licensor(s) grant no additional license rights other than those explicitly granted herein, and except for such limited license rights, all other intellectual property and proprietary rights in and to ʴǰ™ products are and shall remain the exclusive property of ϳԹ and its licensors. The purchaser of ʴǰ™ products agrees to prevent unauthorized use, access, copying, or disclosure of any intellectual property comprised in ʴǰ™ products. The purchaser shall not, and shall not permit anyone to copy, create any derivative work, reverse engineer, disassemble, decompile, or make improvements to ʴǰ™ products. Users and purchasers of ʴǰ™ products hereby grant to ϳԹ exclusive ownership and all rights, title, and interest in and to any improvements made, by purchasers or users, to the ʴǰ™ products (including any improvements by purchasers or users to the intellectual property directly related to ʴǰ™ products), provided however that the user or purchaser who makes the improvement retains a limited, non-transferable license to use such improvement for its internal, non-commercial research use, in accordance with this agreement, only so long as such user or purchaser is in compliance with this agreement. All data, information, and results (to the extent they do not constitute improvements of ʴǰ™ products) entered, stored, compiled, generated, and/or analyzed by purchasers of ʴǰ™ products using the ʴǰ™ products in accordance with all of the foregoing shall be the property of such purchaser.
ʴǰ™ Transfection System and ʴǰ™ Delivery Cartridges (“ʴǰ™ products”) are protected by patents in the U.S. and elsewhere. More information can be found at . The list of products and patents contained therein are subject to change as a result of routine prosecution and dependent on the patent life cycle as determined by the laws of each jurisdiction in which a relevant patent is in effect.
Quality Statement:
UNLESS OTHERWISE STATED, PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES. FOR PRODUCT-SPECIFIC COMPLIANCE AND INTENDED USE INFORMATION, REFER TO THE PRODUCT INFORMATION SHEET. GENERAL INFORMATION ON QUALITY AT ϳԹ MAY BE FOUND AT WWW.ϳԹ.COM/COMPLIANCE.
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