±á±ð±è²¹³Ù¾±°ä³Ü±ô³Ùâ„¢ - Hepatic Organoid Culture Media
±á±ð±è²¹³Ù¾±°ä³Ü±ô³Ùâ„¢ Hepatic Organoid Culture
Complete, Defined Medium for Generating Hepatic Organoids
Using Organoids as Model Systems
Growing hepatic progenitors as organoid cultures enables convenient in vitro characterization of the hepatic epithelium in a physiologically relevant system. In this webinar, Dr. Meritxell Huch discusses organoid culture and their applications in modeling the GI tract.
Why Use ±á±ð±è²¹³Ù¾±°ä³Ü±ô³Ùâ„¢?
- In vitro system for generating organoids within a week.
- No injury models, hand-picking of ducts or cell sorting required.
- Serum-free and defined medium formulation.
- Organoids can be grown from duct fragments or single cells and be cultured in matrix domes or suspension.
Products for Hepatic Organoid Culture
±á±ð±è²¹³Ù¾±°ä³Ü±ô³Ùâ„¢ Organoid Kit (Human)
Recommended for:
Species:
For Use With:
±á±ð±è²¹³Ù¾±°ä³Ü±ô³Ùâ„¢ Organoid Growth Medium
Recommended for:
Species:
For Use With:
Mouse Hepatic Organoids
Recommended for:
Species:
Contents:
Brand History
Despite the remarkable regenerative capacity of the liver in vivo, sustained in vitro culture of liver tissues has remained difficult until recently. In 2013, described a robust culture system for the maintenance of hepatic tissues as organoids. In 2014, Dr. Hans Clevers and the Foundation Hubrecht Organoid Technology signed an agreement with ºÚÁÏ³Ô¹Ï to manufacture and distribute cell culture media for organoids. ±á±ð±è²¹³Ù¾±°ä³Ü±ô³Ùâ„¢ Organoid Growth Medium (Mouse) was released in early 2018, providing researchers with a convenient method for establishing and maintaining hepatic progenitor organoids.
Key Applications of Hepatic Organoids
Adult-Tissue Derived Liver Organoids
Broutier L, et al. (2016) Nat Protoc. 11(9): 1724-43.
Huch M, et al. (2015) Cell. 160(1-2): 299-312.
Huch M, et al. (2013) Nature. 494(7436): 247-50.
Kuijk EW, et al. (2016) Sci Rep. 6: 22154.
Nantasanti S, et al. (2015) Stem Cell Reports. 5(5): 895-907.
Disease Modeling and Cancer Research
Lampis A, et al. (2017) Gastroenterology. S0016-5085(17): 36331-X.
Broutier L, et al. (2017) Nat Med. 23(12): 1424-1435.
Sampaziotis F, et al. (2015) Nat Biotechnol. 33(8): 845-852.
Hindley CJ, et al. (2016) Dev Biol. 420(2): 251-261.
Hepatic Cell Biology
Dorrell C, et al. (2014) Stem Cell Res. 13(2): 275-83.
Tarlow BD, et al. (2014) Hepatology. 60(1): 278-89.
Yimlamai D, et al. (2014) Cell. 157(6): 1324-38.
Kruitwagen HS, et al. (2018) Stem Cells Dev.
hPSC-Derived Liver Organoids
Guye P, et al. (2016) Nat Commun. 7: 10243.
Takebe T, et al. (2017) Cell Rep. 21(10):2661-2670.
Takebe T, et al. (2014) Nat Protoc. 9(2): 396-409.
Organoids in Multi-Lineage Model Systems
Kasuya J, et al. (2015) J Tissue Eng Regen Med. 9(3): 247-56.
Takebe T, et al. (2012) Transplant Proc. 44(4): 1018-20.
Skardal A, et al. (2015) Ann Biomed Eng. 43(10): 2361-73.
