²Ñ±ð²õ±ð²Ô°ä³Ü±ô³Ùâ„¢ Mesenchymal Stromal Cell (MSC) Culture
²Ñ±ð²õ±ð²Ô°ä³Ü±ô³Ùâ„¢ Mesenchymal Stromal Cell Culture
Your High-Performance System for MSC Isolation, Culture & Differentiation
²Ñ±ð²õ±ð²Ô°ä³Ü±ô³Ùâ„¢ Workflow for MSC Derivation, Culture & Differentiation
Derive, culture, expand, differentiate, and cryopreserve MSCs using ²Ñ±ð²õ±ð²Ô°ä³Ü±ô³Ùâ„¢. The ²Ñ±ð²õ±ð²Ô°ä³Ü±ô³Ùâ„¢ product line offers a range of animal component-free and human platelet lysate- and serum-containing media for MSC research.
Why Use ²Ñ±ð²õ±ð²Ô°ä³Ü±ô³Ùâ„¢?
- Species-optimized media formulations provide superior performance compared to traditional and competitor media.
- A comprehensive and integrated range of products is available for the enrichment, expansion, quantification (CFU-F Assay) and differentiation of human and mouse mesenchymal stem cells.
- Standardized MSC culture media and conditions minimize variability and lead to increased reproducibility between experiments.
- Isolate, culture, cryopreserve and differentiate human MSCs with animal component-free, serum-free formulations.
²Ñ±ð²õ±ð²Ô°ä³Ü±ô³Ùâ„¢ Human MSC Media
Animal Component-Free Media
Mesenchymal stem cells (MSCs) have potential utility in a range of cellular therapies, with applications relating to tissue engineering and regenerative medicine, and as vehicles for gene therapy. The limited global supply of fetal bovine serum (FBS) and concerns about immune rejection of transplanted cells or disease transmission make animal component-free media an attractive alternative when expanding cells to numbers for therapeutic purposes.
²Ñ±ð²õ±ð²Ô°ä³Ü±ô³Ùâ„¢-ACF media together allow for the optimized isolation, clonogenic growth, cryopreservation, long-term expansion and differentiation of human MSCs derived from primary tissues (bone marrow and adipose). To date, this is the only complete and integrated culture system completely free of serum and other materials derived from human or animal sources. With animal component-free media, experimental variability is minimized, and the safety concerns associated with exposure to animal-derived materials in translational research are addressed.
²Ñ±ð²õ±ð²Ô°ä³Ü±ô³Ùâ„¢-ACF Plus Medium
Product Type:
Applications:
- MSC isolation and expansion from primary human bone marrow or adipose tissue as well as culture-expanded cells
- Long-term culture of MSCs (>8 passages)
- Detection and enumeration of CFU-F from primary human bone marrow or adipose tissue
Features/Advantages:
- Animal component-free
- Optimized for isolation of MSC directly from primary human tissue
- Superior cell expansion compared to serum-containing medium
- Significant reduction in hematopoietic cell contamination at early passages
- Supports efficient clonogenic growth and long-term expansion of human MSCs
- No adaptation required when transitioning MSCs from serum-containing medium
²Ñ±ð²õ±ð²Ô°ä³Ü±ô³Ùâ„¢-ACF Freezing Medium
Product Type:
Applications:
Features/Advantages:
- Serum-free and animal component-free
- Reproducibly high recovery rates
- Preserves human MSC multipotency and expansion capacities
- Convenient, ready-to-use format
- Optimized for MSCs previously expanded in ²Ñ±ð²õ±ð²Ô°ä³Ü±ô³Ùâ„¢-ACF Plus Medium, ²Ñ±ð²õ±ð²Ô°ä³Ü±ô³Ùâ„¢-hPL Medium, or ²Ñ±ð²õ±ð²Ô°ä³Ü±ô³Ùâ„¢ Proliferation Kit (Human)
²Ñ±ð²õ±ð²Ô°ä³Ü±ô³Ùâ„¢-ACF Chondrogenic Differentiation Medium
Product Type:
Applications:
Features/Advantages:
- Animal component-free (ACF) formulation
- Robust chondrogenic differentiation with as few as 3 x105 MSCs per 3D pellet and as early as day 14
- Strong expression of chondrogenic transcripts - Sox9, Acan, Col2a and Col10a; low expression of hypertrophic transcript Mmp13
- Completes optimized ACF workflow for MSC isolation, expansion, cryopreservation and chondrogenic differentiation when used with other ²Ñ±ð²õ±ð²Ô°ä³Ü±ô³Ùâ„¢-ACF media
- Compatible with MSCs previously expanded in ²Ñ±ð²õ±ð²Ô°ä³Ü±ô³Ùâ„¢-ACF Plus Medium, ²Ñ±ð²õ±ð²Ô°ä³Ü±ô³Ùâ„¢-hPL Medium, or ²Ñ±ð²õ±ð²Ô°ä³Ü±ô³Ùâ„¢ Proliferation Kit (Human)
Explore More ²Ñ±ð²õ±ð²Ô°ä³Ü±ô³Ùâ„¢ Products for Human MSCs
Find more products for your human MSC research, including ²Ñ±ð²õ±ð²Ô°ä³Ü±ô³Ùâ„¢ Osteogenic Differentiation Kit for the differentiation of MSCs into osteoblasts, ²Ñ±ð²õ±ð²Ô°ä³Ü±ô³Ùâ„¢ Adipogenic Differentiation Kit for the differentiation of MSCs into adipocytes, and ²Ñ±ð²õ±ð²Ô°ä³Ü±ô³Ùâ„¢ Proliferation Kit for the detection of CFU-F.
²Ñ±ð²õ±ð²Ô°ä³Ü±ô³Ùâ„¢ Mouse MSC Media
²Ñ±ð²õ±ð²Ô°ä³Ü±ô³Ùâ„¢ Expansion Kit (Mouse)
Product Type:
Applications:
- Derivation and expansion of mouse MSCs and MEFs
- Enumeration of mouse MSCs and MEFs using the CFU-F assay
Features/Advantages:
- Fast expansion of mouse MSCs with robust enrichment as early as passage 0
- Optimized for use with mouse bone marrow-, compact bone- and adipose-derived MSCs and MEFs
- Obtain homogeneous mouse MSC cultures while maintaining tri-lineage differentiation potential
- Rigorous raw material screening and quality control minimize lot-to-lot variability
²Ñ±ð²õ±ð²Ô°ä³Ü±ô³Ùâ„¢ Osteogenic Stimulatory Kit (Mouse)
Product Type:
Applications:
- In vitro differentiation of mouse MSCs and mouse embryonic fibroblasts (MEFs) into osteoblasts
- Bone development studies
Features/Advantages:
- Achieve unparalleled levels of osteogenic differentiation compared to competitor formulations
- Superior expression of key osteogenic transcripts involved in bone differentiation and maturation
- Robust bone matrix deposition (Alizarin Red staining)
²Ñ±ð²õ±ð²Ô°ä³Ü±ô³Ùâ„¢ Adipogenic Differentiation Kit (Mouse)
Product Type:
Applications:
- In vitro differentiation of mouse mesenchymal stem and progenitor cells (MSCs), adipose tissue-derived MSCs (ADSCs), and mouse embryonic fibroblasts (MEFs) into cells of the adipogenic lineage
Features/Advantages:
- Compatible with mouse MSCs previously culture-expanded using the ²Ñ±ð²õ±ð²Ô°ä³Ü±ô³Ùâ„¢ Expansion Kit (Mouse).
- Available in an easy-to-use two-component format.
- Rigorous raw material screening and quality control minimize lot-to-lot variability.
Scientific Resources
How to Create a Hypoxic Environment for Cell Culture
Learn how to mimic physiological conditions to support MSC growth in vitro with a Hypoxia Incubator Chamber.
MSC Survey Results
Read what 450 researchers from around the world had to say in our survey on the state of the MSC field.
Key Applications
Serum-Free Culture of Human Bone-Marrow Derived Mesenchymal Stem Cells
Serum-Free Culture of Human Adipose-Derived Mesenchymal Stem Cells
Other Uses
3D Bioreactor Culture Systems for Mesenchymal Stem Cells
Tan KY et al. (2015) Cytotherapy 17(8): 1152-65
Hervy M et al. (2014) PLoS One. 9(3):e92120
Serum-Free Culture of Human Umbilical Cord-Derived Mesenchymal Stem Cells
Chen G et al. (2014) PLoS One 9(6):e98565
Chen GC et al. (2014) Cell Tissue Bank 15(4): 513-21
Zhou P et al. (2013) Zhongquo Shi Yan Xue Ye Xue Za Zhi 21(5): 1256-60
Serum-Free Culture of Human Placenta-Derived Mesenchymal Stem Cells
