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The Mouse Interleukin 12/23 (IL-12/-23) p40 ELISA Kit is designed for the quantitative detection and measurement of mouse IL-12 and IL-23 in biological fluids such as serum, plasma, and cell culture supernatants. IL-12 and IL-23 are heterodimeric cytokines that share a common p40 subunit. IL-12 is produced by monocytes, macrophages, dendritic cells, neutrophils, and B cells. IL-12 is a regulator of cytokine synthesis, especially IFN-γ, enhances the proliferation of T and NK cells, and stimulates the differentiation of CD4+ and CD8+ T cells. IL-23 is secreted by activated macrophages and dendritic cells, and induces the proliferation of memory T cells and the production of IL-17.
The assay is based on the sandwich ELISA method, in which samples are added to ELISA strip plates pre-coated with capture antibodies specific for the cytokine. The captured cytokine is detected by addition of a biotinylated detection antibody, followed by streptavidin-horseradish peroxidase, which binds the biotinylated antibody. Addition of the chromogenic enzyme substrate 3,3’,5,5’ tetramethylbenzidine (TMB) results in a colored product with an intensity directly proportional to the concentration of cytokine in the sample. The concentration of the cytokine is determined by comparison to a serial dilution of the cytokine standard analyzed in parallel.
Subtype
Complete Kits
Cell Type
B Cells, Dendritic Cells, Granulocytes and Subsets, Macrophages, Monocytes
• Reportable range: 10 - 3160 pg/mL. This is the concentration range in which measurement of the analyte can be done with the highest precision, accuracy, and linearity.
• Sensitivity: The limit of detection of this assay is 8 pg/mL. This is the analyte concentration with absorbance two standard deviations higher than the zero standard.
• Accuracy: No international standard exists for calibration.
• Recovery: A mid-curve recovery of 87 - 117% was determined by spiking defined amounts of analyte standard into serum or plasma samples in repeated experiments.
• Precision: The intra-assay precision of this assay is 2.9% (CV). The inter-assay precision of this assay is 14.1% (CV).
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