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The RosetteSep? Human Cord Blood Progenitor Cell Enrichment Cocktail with HetaSep? is designed to isolate progenitor cells from cord blood by negative selection. The HetaSep? minimizes the required volume of the RosetteSep? cocktail reagent by first concentrating the sample by sedimentation with HetaSep? (Catalog #07906), a solution of hetastarch. The hematopoietic progenitors in the resultant buffy coat are then enriched using a modified RosetteSep? protocol that uses less RosetteSep? cocktail than is required for an unconcentrated sample. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing CD2, CD3, CD14, CD16, CD19, CD24, CD56, CD61, CD66b and glycophorin A on red blood cells (RBCs). When centrifuged over a density gradient medium the unwanted cells pellet along with the RBCs. The purified progenitor cells are present as a highly enriched population at the interface between the plasma and the density gradient medium.

We regret to inform our customers that we can no longer include Ficoll-Paque? Plus with this product. For performing cell isolation by density gradient centrifugation, we recommend purchasing Lymphoprep? (a href="/products/lymphoprep.html">Catalog #18060), which is available in a variety of sizes and formats. As with Ficoll-Paque?, Lymphoprep? has a density of 1.077 g/mL, and after centrifugation, mononuclear cells remain at the plasma-Lymphoprep? interface while granulocytes and erythrocytes sediment below.