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Meniscus-free cultureware for more accurate counting of hematopoietic colonies in CFU assays

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Meniscus-free cultureware for more accurate counting of hematopoietic colonies in CFU assays

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Meniscus-free cultureware for more accurate counting of hematopoietic colonies in CFU assays
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Overview

Achieve accurate and reproducible hematopoietic colony counting with ³§³¾²¹°ù³Ù¶Ù¾±²õ³óâ„¢ plates. These plates prevent the formation of a meniscus, allowing for even distribution of culture medium, and resulting in a more uniform distribution of colonies throughout the entire 35 mm well. The absence of the meniscus also reduces optical distortion so that colonies located at the edge can be more easily counted.

³§³¾²¹°ù³Ù¶Ù¾±²õ³óâ„¢ (Catalog #27370) is the second generation of the original ³§³¾²¹°ù³Ù¶Ù¾±²õ³óâ„¢ plate (Catalog #27301). These new plates use an improved coating, which streamlines the manufacturing process, while consistently improving the visibility of hematopoietic colonies in culture through the elimination of the meniscus. Current ³§³¾²¹°ù³Ù¶Ù¾±²õ³óâ„¢ customers can contact their local ºÚÁÏ³Ô¹Ï representative for more information.

³§³¾²¹°ù³Ù¶Ù¾±²õ³óâ„¢ has been designed for both manual counting with STEMgridâ„¢-6 (Catalog #27000), as well as automated colony counting with a STEMvisionâ„¢ instrument (Catalog #22000). Browse our Frequently Asked Questions (FAQs) on performing the CFU assay.
Subtype
Dishes and Plates
Species
Human, Mouse, Non-Human Primate, Other, Rat
Application
Cell Culture, Colony Assay
Brand
SmartDish

Data Figures

Manual and Automated Counting of Hematopoietic CFU Assays

Figure 1. Manual and Automated Counting of CFU Assays in Standard and SmartDish™ Culture Plates

Shown are representative 35 mm wells from (A) standard and (B) ³§³¾²¹°ù³Ù¶Ù¾±²õ³óâ„¢ culture plates with STEMgrid-6 attached to facilitate colony counting on an inverted microscope, or (C) standard and (D) ³§³¾²¹°ù³Ù¶Ù¾±²õ³óâ„¢ plates imaged using STEMvisionâ„¢. Optical distortion caused by the meniscus obscures colonies at the edge of cultures grown in standard plates (A, C) and makes them more difficult to count (see circled colony in A). Colonies are more easily seen and counted at the edge of the ³§³¾²¹°ù³Ù¶Ù¾±²õ³óâ„¢ wells which have been treated to eliminate meniscus formation (B, D).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Name
Catalog #
27371
Lot #
All
Language
English
Document Type
Product Name
Catalog #
27370
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.