Hematopoietic Stem and Progenitor Cell Research
The defining properties of multipotent hematopoietic stem cells (HSCs) are their ability to differentiate into multiple lymphoid and myeloid lineages, as well as to self-renew to ensure that the HSC pool is not depleted over the lifespan of an individual. As HSCs proliferate, they progress through a series of lineage commitment steps, producing hematopoietic progenitor cells (HPCs) that are shorter-lived and more restricted in their differentiation potential.
The screening of novel compounds to predict potential toxicity to the hematopoietic system (i.e. hematotoxicity) is an important step in drug development. HemaTox™ assays can assess the toxicity of drugs on the growth and lineage-specific differentiation of human CD34+ hematopoietic stem and progenitor cells (HSPCs) into one of three specific progenitor cell lineages (erythroid, myeloid, or megakaryocyte).
Explore our scientific resources below to learn more about how to source, culture, and analyze HSPCs.
Procedure for Setting Up the CFU Assay
This video provides step-by-step guidelines for setting up hematopoietic colony-forming unit (CFU) assays using methylcellulose-based MethoCult™ medium. Steps include preparing cells, plating semi-solid medium and incubating.
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How to Prepare and Plate Semi-Solid MediumSteps involved with preparing and plating methylcellulose-based semi-solid media (e.g. MethoCult™ and ClonaCell™) -
New Tools for the Ex Vivo Expansion of Human Hematopoietic Stem and Progenitor CellsThe development of standardized ex vivo HSPC expansion methods is needed to generate clinically relevant cell numbers for transplantation. This webinar will review key concepts in human HSPC biology, including standardized methods and recently demonstrated strategies for expanding HSPCs in culture. Additionally, ϳԹ products for the entire workflow of HSPC expansion will be covered, from isolation to expansion and differentiation, and analysis. -
A Guide to Erythropoiesis - EPO Signaling and Erythroid DevelopmentGet an overview of erythroid development and erythropoietin (EPO) signaling pathways in this on-demand webinar, and explore methods to measure EPO activity and screening for EPO mimetics. -
Assays for Hematopoietic Stem and Progenitor Cells in Cord BloodThis webinar, by Dr. Bert Wognum, will provide an overview of the value of assessing hematopoietic progenitor cell content in cord blood. In addition to reviewing the current assays used, new methods incorporating automation will be discussed.
