Reprogramming of Pluripotent Stem Cells
Human induced pluripotent stem (hiPS) cells are generated by reprogramming somatic cells to a pluripotent state, through the transient overexpression of key reprogramming factors.
Explore the resources below for information on the reprogramming of hPSCs.
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ReproRNA™-OKSGM: Reprogramming Vector for Human iPS Cell Generation
This technical video demonstrates how to reprogram fibroblasts in ReproTeSR™ medium using ReproRNA™-OKSGM. ReproRNA™-OKSGM is a non-integrating, self-replicating RNA reprogramming vector for generating human induced pluripotent (iPS) cells.
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iPSCs As Models, Part 1: What Are Induced Pluripotent Stem Cells and How Do You Use Them?Hear from Dr. Anjana Nityanandam, Director of the Human Stem Cell Lab core facility at St. Jude Children’s Research Hospital, as she provides some introductory examples of how iPSCs are used for disease modeling and walks through how to generate and characterize iPSCs. Learn about quality control measures to improve reliability and reproducibility of data, and how to address interline variability in your experiments. -
Extending the Life of a CFU AssayTechnical tip from our dedicated team of Product and Scientific Support specialists -
How to Reprogram Fibroblasts into Human Induced Pluripotent Stem (iPS) Cells Using ReproRNA™-OKSGMHow to reprogram fibroblasts in using ReproRNA™-OKSGM is a non-integrating, self-replicating RNA reprogramming vector for generating human induced pluripotent stem (iPS) cells -
Small Molecules, Big Impact in Pluripotent Stem Cell ResearchOverview of signaling pathways and small molecules in pluripotent stem cell research -
Tips for Reprogramming Somatic Cells using TeSR™-E7™Tips to help resolve 2 common issues encountered when reprogramming fibroblasts using TeSR™-E7™ -
Optimizing the Puromycin Selection Step in our ReproRNA™-OKSGM WorkflowTechnical tip from our dedicated team of Product and Scientific Support specialists -
How to Harvest Reprogrammed iPS Cell Colonies in TeSR™-E7™ Reprogramming Culture MediumCharacteristics of reprogrammed iPS cell colonies and provides instructions for manually harvesting putative iPS cell colonies for replating and further expansion
