Airway Modeling
Physiologically relevant in vitro lung models that closely resemble the in vivo human airway are critical for enabling pulmonary research. Therefore specialized cell culture techniques have been developed to recapitulate the morphological and functional characteristics of the pseudostratified epithelium, at the air-liquid interface (ALI) or as airway organoids. This has expanded the range of applications and relevance of in vitro culture systems.
Below is a collection of resources to help with your airway modeling for pulmonary research.
How to Model the Human Airway at the Air-Liquid Interface
Primary human bronchial epithelial cells (HBECs) can be expanded and differentiated into a pseudostratified mucociliary epithelium that closely mimics what is observed in vivo. Watch this protocol video to learn more.
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How to Model the Human Airway at the Air-Liquid Interface: Expansion of HBECsLearn how to expand human bronchial epithelial cells (HBECs) to initiate an air-liquid interface (ALI) culture using PneumaCult™ culture media -
How to Perform Immunocytochemistry (ICC) Staining of Epithelial Cells Cultured as Monolayers or at the Air-Liquid InterfaceHow to perform immunocytochemistry (ICC) staining of air-liquid interface or monolayer cultures in your own lab -
Why Use Air-Liquid Interface Cultures for Respiratory Research: Q&A with Dr. WadsworthDr. Wadsworth discusses the features, benefits & applications of ALI culture & explains how this technique contributes towards his research -
How to Model the Human Airway at the Air-Liquid Interface: Differentiation of HBECsLearn how to differentiate human bronchial epithelial cells (HBECs) at the air-liquid interface (ALI) using PneumaCult™ culture media -
How to Model the Human Airway at the Air-Liquid Interface: IntroductionProtocols for expanding and differentiating human bronchial epithelial cells (HBECs) at the air-liquid interface (ALI) using the PneumaCult™ culture system to demonstrate ALI culture -
Air-Liquid Interface (ALI) Culture of Bronchial Epithelial Cells with PneumaCult™-ALI MediumPneumaCult™-ALI is a serum- and BPE-free medium for the culture of primary human bronchial epithelial cells (HBECs) at the ALI
