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EasySep™ Direct HLA B Cell Isolation Kit

Immunomagnetic negative selection of untouched human B cells from whole blood, buffy coat, spleen, or lymph nodes

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New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more

EasySep™ Direct HLA B Cell Isolation Kit

Immunomagnetic negative selection of untouched human B cells from whole blood, buffy coat, spleen, or lymph nodes

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Immunomagnetic negative selection of untouched human B cells from whole blood, buffy coat, spleen, or lymph nodes
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What's Included

  • EasySep™ Direct HLA B Cell Isolation Kit (Catalog #89684)
    • EasySep™ Direct HLA B Cell Isolation Cocktail, 2 x 2.5 mL
    • EasySep™ Direct RapidSpheres™ 50302, 4 x 2.5 mL
  • EasySep™ Direct HLA B Cell Isolation Kit for Dzdz™ (Catalog #89684RF)
    • EasySep™ Direct HLA B Cell Isolation Cocktail, 2 x 2.5 mL
    • EasySep™ Direct RapidSpheres™ 50302, 4 x 2.5 mL
    • Dzdz™ Buffer (Catalog #20104)
    • Dzdz™ Filter Tips (Catalog #20125)
    
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. EasySep™ Magnet
    EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

  2. D-PBS (Without Ca++ and Mg++)
    D-PBS (Without Ca++ and Mg++)

    Dulbecco’s phosphate-buffered saline without calcium and magnesium

Overview

Easily and efficiently isolate highly purified human B cells directly from human whole blood, buffy coat, spleen, or lymph node samples by immunomagnetic negative selection, with the EasySep™ Direct HLA B Cell Isolation Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep™ negative selection procedure, unwanted cells are labeled with antibody complexes and magnetic particles called EasySep™ Direct RapidSpheres™. Unwanted cells expressing the following markers are targeted for removal: CD2, CD3, CD15, CD16, CD36, CD43, CD56, and CD66b. The magnetically labeled cells are then separated from the untouched desired B cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube. Following magnetic cell isolation, the desired B cells are ready for downstream applications such as flow cytometry crossmatch.

Please Note: In light of the new EU Regulation 2017/746 (IVDR) of the European Parliament and of the Council on in vitro diagnostic medical devices, which became effective in 2022, ϳԹ deregistered and removed the CE IVD claims associated with these products, and they are now labeled “For Research Use Only”. Please note there have been no changes to the form, function, and quality assurances associated with these products. For more information, please contact the Quality Assurance and Regulatory Department at qaschangenote@stemcell.com.

The EasySep™ Direct HLA B Cell Isolation Kit (Catalog #89684) is available for sale in Canada, the EU, UK, and Australia. In all other regions, the EasySep™ Direct HLA Crossmatch B Cell Isolation Kit (Catalog #19684) is available as an equivalent product.

Learn more about how immunomagnetic EasySep™ technology works or how to fully automate immunomagnetic cell isolation with Dzdz™ to save time and increase laboratory throughput. Explore additional products optimized for your workflow, including those for cell characterization, cryopreservation, and more.

Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• Easy 50 EasySep™ Magnet (Catalog #18002)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• Dzdz™-S (Catalog #20001)
Subtype
Cell Isolation Kits
Cell Type
B Cells
Species
Human
Sample Source
Lymph Node, Spleen, Whole Blood
Selection Method
Negative
Application
Cell Isolation
Brand
EasySep
Area of Interest
HLA, Immunology

Data Figures

Typical EasySep™ Direct HLA B Cell Isolation Profile

Figure 1. Typical EasySep™ Direct HLA B Cell Isolation Profile

Starting with human whole blood from normal healthy donors, the B cell (CD19+) content of the non-lysed final isolated fraction typically ranges from 89 - 99.8%. In the above example, the B cell (CD19+) content of the lysed whole blood start sample and non-lysed final isolated fraction is 12.3% and 98.4% (gated on CD45), respectively.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Name
Catalog #
89684
Lot #
All
Language
Multi
Document Type
Product Name
Catalog #
89684
Lot #
All
Language
English
Document Type
Product Name
Catalog #
89684RF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
89684RF
Lot #
All
Language
Multi
Document Type
Product Name
Catalog #
89684
Lot #
All
Language
English
Document Type
Product Name
Catalog #
89684
Lot #
All
Language
English
Document Type
Product Name
Catalog #
89684RF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
89684RF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
89684RF
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (11)

Brochure
Brochure
Brochure
Cell Separation Solutions for the Flow Cytometric Crossmatch Assay
Technical Bulletin
Cell Separation Solutions for the Flow Cytometric Crossmatch Assay
Frequencies of Human Cell Types in Blood-Related Sources
Wallchart
Frequencies of Human Cell Types in Blood-Related Sources
Human Immune Cytokines
Wallchart
Human Immune Cytokines
How to Prepare a Buffy Coat from Whole Blood
3:46
Video
How to Prepare a Buffy Coat from Whole Blood
Automate Cell Isolation with the Dzdz™-S Cell Separation Instrument
1:41
Video
Automate Cell Isolation with the Dzdz™-S Cell Separation Instrument
Cell Isolation Directly from Whole Blood without RBC Lysis or Centrifugation: EasySep™ Direct
1:44
Video
Cell Isolation Directly from Whole Blood without RBC Lysis or Centrifugation: EasySep™ Direct
Scientific Poster
Scientific Poster

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New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more
Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT ϳԹ, REFER TO WWW.ϳԹ.COM/COMPLIANCE.

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