EasySep™ Direct HLA T Cell Isolation Kit
Immunomagnetic negative selection of untouched human T cells from whole blood, buffy coat, spleen, or lymph nodes

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In-lab demonstration
- EasySep™ Magnet
Magnet for column-free immunomagnetic separation
- D-PBS (Without Ca++ and Mg++)
Dulbecco’s phosphate-buffered saline without calcium and magnesium
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Labeling Antibodies
Compatible antibodies for purity assessment of isolated cells
Overview
In this EasySep™ negative selection procedure, unwanted cells are labeled with antibody complexes and magnetic particles called EasySep™ Direct RapidSpheres™. The following unwanted cells are targeted for removal: B cells, NK cells, monocytes, neutrophils, eosinophils, and platelets. The magnetically labeled cells are then separated from the untouched desired T cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube. Following magnetic cell isolation, the desired T cells are ready for downstream applications such as flow cytometry crossmatch.
Please Note: In light of the new EU Regulation 2017/746 (IVDR) of the European Parliament and of the Council on in vitro diagnostic medical devices, which became effective in 2022, ϳԹ deregistered and removed the CE IVD claims associated with these products, and they are now labeled “For Research Use Only”. Please note there have been no changes to the form, function, and quality assurances associated with these products. For more information, please contact the Quality Assurance and Regulatory Department at qaschangenote@stemcell.com.
The EasySep™ Direct HLA T Cell Isolation Kit (Catalog #89671) is available for sale in Canada, the EU, UK, and Australia. In all other regions, The EasySep™ Direct HLA Crossmatch T Cell Isolation Kit (Catalog# 19671) is available as an equivalent product.
Learn more about how immunomagnetic EasySep™ technology works or how to fully automate immunomagnetic cell isolation with Dzdz™ to save time and increase laboratory throughput. Explore additional products optimized for your workflow, including those for cell characterization, cryopreservation, and more.
Data Figures

Figure 1. Typical EasySep™ Direct HLA T Cell Isolation Profile
Starting with human whole blood from normal healthy donors, the T cell (CD3+) content of the non-lysed final isolated fraction typically ranges from 89 - 99.9% (gated on CD45). In the above example, the T cell (CD3+) content of the lysed whole blood start sample and non-lysed final isolated fraction is 38.0% and 93.2% (gated on CD45), respectively.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (10)
Related Products
- EasySep™ Human T Cell Isolation Kit
Immunomagnetic negative isolation of untouched human T cells
- Dzdz™-S
Fully automated cell separator
- Human Whole Peripheral Blood
Primary human cells, fresh
- EasySep™ Direct HLA B Cell Isolation Kit
Immunomagnetic negative selection of untouched human B cells from whole blood, buffy coat, spleen, or lymph nodes
Labeling Antibodies
- Anti-Human CD3 Antibody, Clone UCHT1
Mouse (BALB/c) monoclonal IgG1 antibody against human, chimpanzee CD3
- Anti-Human CD45 Antibody, Clone HI30
Mouse monoclonal IgG1 antibody against human, chimpanzee CD45
- Anti-Human CD3 Antibody, Clone SK7
Mouse monoclonal antibody against human, chimpanzee CD3
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EasySep™ Direct HLA T Cell Isolation Kit
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT ϳԹ, REFER TO WWW.ϳԹ.COM/COMPLIANCE.