´¡²µ²µ°ù±ð°Â±ð±ô±ôâ„¢ Microwell Plates - Spheroids and Embryoid Bodies
AggreWell™ Microwell Plates
Easy, Reproducible 3D Spheroid and Embryoid Body Production
AggreWell™ plates contain microwells that are 400 µm (AggreWell™400), 800 µm (AggreWell™800) or 900 µm (´¡²µ²µ°ù±ð°Â±ð±ô±ôâ„¢HT) in size, providing flexibility to generate spheroids of desired size for your research.
How Does AggreWell™ Work?
Watch this short video to learn more about how AggreWell™ works to generate uniformly-sized and shaped 3D spheroids and embryoid bodies.
To produce spheroids or embryoid bodies using AggreWell, simply add a single cell suspension to the microwells, and then centrifuging to distribute the cells evenly in the microwells. Incubate overnight and assess the spheroids after 24-48 hours. Then culture in the microwells, or harvest and perform downstream assays.
Why Use AggreWell™ to Generate EBs and Spheroids?
- Use a simple protocol to generate spheroids and EBs.
- Produce large numbers of uniform spheroids, with consistent size and shape.
- Control the size of the spheroids by modifying the cell seeding concentration.
- Obtain up to 5,900 spheroids per well.
- Scale up production of spheroids and EBs from a single plate for a fraction of a penny per spheroid.
AggreWell™400
Microwell Size:
Cell Range:
Plate Formats Available:
6-well plate, 1,500 aggregates per well
Applications:
- Embryoid body for ES and iPS cell directed differentiation
- Cancer spheroid cultures
- Drug discovery
- 3D tissue engineering
- Imaging spheroids
AggreWell™800
Microwell Size:
Cell Range:
Plate Formats Available:
6-well plate, 5,900 aggregates per well
Applications:
- Embryoid body for ES and iPS cell directed differentiation
- Cancer spheroid cultures
- Drug discovery
- 3D tissue engineering
- Imaging spheroids
AggreWell™HT
Microwell Size:
Cell Range:
Plate Formats Available:
Applications:
- Embryoid body for ES and iPS cell directed differentiation
- Cancer spheroid cultures
- Drug discovery
- 3D tissue engineering
- Imaging spheroids
- Drug Screening
Scientific Resources
Key Applications and Related Publications
ES and iPS Cell Directed Differentiation (Selected)
Cheung et al. (2014) Stem Cell Reports 2(4):534-46
Hartjes et al. (2014) Stem Cells 32(9):2350-9
Jang et al. (2014) J Stem Cell Res Ther 4(2):174
Kinney et al. (2014) Sci Rep 4: 4290
Kokkinaki M. (2011) STEM CELLS 29:825–835
Nguyen et al. (2014) Stem Cell Reports 3(2): 260–268
Sebastiano V et al. (2014) Sci Transl Med 6(264):264ra163
Ungrin et al. (2012) Biotechnol Bioeng 109(4): 853-66
van Wilgenburg et al. (2013) PloS one 8(8):e71098
Cancer Spheroid Research
Razian et al. (2013) J Vis Exp. 81: 50665
Wrzesinski et al. (2014) PLoS ONE 9(9): e106973
Drug Screening & Drug Delivery
Bratt-Leal A et al. (2013). Biomaterials 34(30): 7227-35
Fey et al. (2012) Toxicol Sci 127(2): 403-11
Lim et al. (2011) Acta Biomater 7(3): 986-95
Lei et al. (2014) Biomater Sci 2: 666-673
Disease Modeling
Aflaki et al. (2014) Sci Transl Med. 11;6(240): 240ra73
Moya et al. (2013) Stem Cell Research & Therapy, 4(Suppl 1):S15
3D Tissue Engineering
Cho et al. (2013) Biomaterials 34(3): 651-61
Cimetta et al. (2014) Exp Biol Med. 239(9):1255-63
Kabiri et al. (2012) Biochem Biophys Res Commun 419(2): 142-7
Suspension Culture of MSCs
Baraniak et al. (2012) . Cell Tissue Res. 347(3): 701-11
Cook et al. (2012) . Tissue Engineering Part C: Methods 18(5): 319-328
Rettinger et al. (2014) Cell Tissue Res 358(2): 395-405
Additional Applications
Wallace et al. (2013) Methods Mol Biol 989: 153-64
AggreWell™ Background
Ungrin et al. (2008) PLoS One 3(2) e1565
