´¡³¢¶Ù·¡¹ó³¢±«°¿¸éâ„¢ Kit for ALDH Assays
ALDEFLUOR™ Kit for ALDH Assays
Identification, Evaluation and Isolation of Normal and Cancer Stem & Progenitor Cells
ALDEFLUOR™ is a non-immunological fluorescent reagent system that has supported over 1000 publications for the detection of aldehyde dehydrogenase-bright (ALDHbr) cells in over 80 different tissues. High expression of ALDH has been reported for normal and cancer stem and progenitor cells of various lineages, including hematopoietic, mammary, endothelial, mesenchymal and neural cells. Only cells with an intact cellular membrane can retain the ALDEFLUOR™ reaction product, making this system selective for viable ALDHbr cells. ALDEFLUOR™ is a non-toxic and easy-to-use kit that requires no antibody staining, and is compatible with standard cell sorters and analyzers.
How ´¡³¢¶Ù·¡¹ó³¢±«°¿¸éâ„¢ Detects Normal and Cancer Progenitor Cells
The ´¡³¢¶Ù·¡¹ó³¢±«°¿¸éâ„¢ kit contains BODIPY-aminoacetaldehyde (BAAA), a fluorescent non-toxic substrate for ALDH, which freely diffuses into intact and viable cells. In the presence of ALDH, BAAA is converted into BODIPY-aminoacetate (BAA), a negatively charged product that is retained inside the cells. Intracellular accumulation of BAA leads to increased fluorescence, and these ALDH-bright (ALDHbr) cells can be analyzed with flow cytometry. Watch this video to learn more about how ´¡³¢¶Ù·¡¹ó³¢±«°¿¸éâ„¢ works.
Why Use ALDEFLUOR™?
- Detect viable normal or cancer progenitor cells based on ALDH activity. No antibody staining required.
- Can be used with multiple cell types and species.
- Identifies only viable cells with an intact cell membrane. Compatible with immunophenotyping.
- Has supported more than 1000 peer-reviewed publications.
- Simple protocol with highly reproducible results. Compatible with standard cell sorters or analyzers.
´¡³¢¶Ù·¡¹ó³¢±«°¿¸éâ„¢ Kit
Recommended for:
Detection of stem and progenitor cells from normal and cancerous tissue
Species:
Human, Mouse, Rat
Related ALDEFLUOR™ Products:
ALDHbr Assay Kit
Recommended for:
Detection of ALDHbrCD34+ hematopoietic stem and progenitor cells in cord blood
Species:
Human
Related ALDEFLUOR™ Products:
ALDEFLUOR™ Assay Buffer
Recommended for:
Counterstaining ALDEFLUOR™-reacted cells with immunofluorescently-labeled antibodies
Species:
Human, Mouse, Rat
Related ALDEFLUOR™ Products:
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Optimizing ´¡³¢¶Ù·¡¹ó³¢±«°¿¸éâ„¢ for Various Tissue Types
´¡³¢¶Ù·¡¹ó³¢±«°¿¸éâ„¢ was initially developed and optimized for the detection of ALDHbr hematopoietic stem and progenitor cells in human blood and bone marrow. Since then it has also been shown to detect normal and neoplastic cells in many other tissue types (including breast, colon, lung, pancreas, and thyroid) as well as cancer cell lines. Optimizing the ´¡³¢¶Ù·¡¹ó³¢±«°¿¸éâ„¢ protocol to your tissue type of interest can dramatically increase the fluorescence intensity, thereby optimizing the assay performance. Watch this video to see an example of how our scientists increased the fluorescence intensity of ALDHbr cells in mammary tissue and learn how you can modify the protocol to fit your research needs.
Related Resources
Brochure
Tools for Cancer Research
Wallchart
SnapShot: Breast Cancer
Technical Bulletin
Identification of ALDH-Expressing Cancer Stem Cells Using ´¡³¢¶Ù·¡¹ó³¢±«°¿¸éâ„¢
Key Applications
Publications on Normal Cells
Hematopoietic Cells
Boxall SA et al. (2008) Bone Marrow Transplant 43(8): 627-35.
Capoccia BJ et al. (2009) Blood 113(21): 5340-51.
Christ O et al. (2007) Haematologica 92(9): 1165-72.
Fallon P et al. (2003) . Br J Haematol 122: 99-108.
Gentry T et al. (2007) Cytotherapy 9(6): 569-76.
Gentry T et al. (2007) . Cytotherapy 9(3): 259-74.
Gündüz E et al. (2010) . Cytotherapy 12(8): 1006-12.
Hess DA et al. (2004) . Blood 104(6): 1648-55.
Hess DA et al. (2008) . Stem Cells 26(3): 611-20.
Liu C et al. (2010) . Blood 116(25): 5518-27.
Muramoto GG et al. (2010) . Stem Cells 28(3): 523-34.
Pearce DJ & Connet D. (2007) . Exp Hematol 35(9): 1437-46.
Pierre-Louis O et al. (2009) Stem Cells 27(10): 2552-62.
Povsic TJ et al. (2009) . J Thromb Thrombolysis 28(3): 259-65.
Povsic TJ et al. (2010) J Gerontol A Biol Sci Med Sci 65(10): 1042-50.
Sondergaard CS et al. (2010) J Transl Med 8: 24.
Storms RW et al. (1999) . Proc Natl Acad Sci U S A 96: 9118-23.
Shoulars K et al. (2016) . Blood 127(19):2346-54.
Capoccia BJ et al. (2009) Blood 113(21): 5340-51.
Christ O et al. (2007) Haematologica 92(9): 1165-72.
Fallon P et al. (2003) . Br J Haematol 122: 99-108.
Gentry T et al. (2007) Cytotherapy 9(6): 569-76.
Gentry T et al. (2007) . Cytotherapy 9(3): 259-74.
Gündüz E et al. (2010) . Cytotherapy 12(8): 1006-12.
Hess DA et al. (2004) . Blood 104(6): 1648-55.
Hess DA et al. (2008) . Stem Cells 26(3): 611-20.
Liu C et al. (2010) . Blood 116(25): 5518-27.
Muramoto GG et al. (2010) . Stem Cells 28(3): 523-34.
Pearce DJ & Connet D. (2007) . Exp Hematol 35(9): 1437-46.
Pierre-Louis O et al. (2009) Stem Cells 27(10): 2552-62.
Povsic TJ et al. (2009) . J Thromb Thrombolysis 28(3): 259-65.
Povsic TJ et al. (2010) J Gerontol A Biol Sci Med Sci 65(10): 1042-50.
Sondergaard CS et al. (2010) J Transl Med 8: 24.
Storms RW et al. (1999) . Proc Natl Acad Sci U S A 96: 9118-23.
Shoulars K et al. (2016) . Blood 127(19):2346-54.
Colon Cells
Carpentino JE et al. (2009). Cancer Res 69(20): 8208-15.
Huang EH et al. (2009) . Cancer Res 69(8): 3382-9.
Huang EH et al. (2009) . Cancer Res 69(8): 3382-9.
Endothelial Cells
Povsic TJ et al. (2009) . Am Heart J 157(2): 335-44.
Lung Cells
Moreb JS et al. (2007) Cytometry B Clin Cytom 72(4): 281-9.
Mammary Cells
Ginestier C et al. (2007) Cell Stem Cell 1(5): 555-67.
Liu S et al. (2008) Proc Natl Acad Sci U S A 105(5): 1680-5.
Liu S et al. (2008) Proc Natl Acad Sci U S A 105(5): 1680-5.
Mesenchymal Cells
Nagano M et al. (2010) Stem Cells Dev 19(8): 1195-210.
Muscle Cells
Jean E et al. (2011) J Cell Mol Med 15(1):119-33.
Vauchez K et al. (2009) Mol Ther 17(11): 1948-58.
Vauchez K et al. (2009) Mol Ther 17(11): 1948-58.
Neural Cells
Cai J et al. (2004) . J Neurochem 88(1): 212-26.
Corti S et al. (2006) . Stem Cells 24(4): 975-85.
Corti S et al. (2006) . Hum Mol Genet 15(2): 167-87.
Corti S et al. (2008) . J Clin Invest 118(10): 3316-30.
Obermair FJ et al. (2010) . Stem Cell Res 5(2): 131-43.
Corti S et al. (2006) . Stem Cells 24(4): 975-85.
Corti S et al. (2006) . Hum Mol Genet 15(2): 167-87.
Corti S et al. (2008) . J Clin Invest 118(10): 3316-30.
Obermair FJ et al. (2010) . Stem Cell Res 5(2): 131-43.
Pancreatic Cells
Rovira M et al. (2010) Proc Natl Acad Sci U S A 107(1): 75-80.
Prostate Cells
Yao M et al. (2010) Cells Tissues Organs 191(3): 203-12.
Publications on Cancer Cells
Cancer Stem Cells: Review Articles
Alison MR et al. (2011) J Pathol 223(2): 147-161.
Alison MR et al. (2010) J Pathol 222(4): 335-44.
Ma I & Allan AL. (2011) Stem Cell Rev & Rep 7(2): 292-306.
Alison MR et al. (2010) J Pathol 222(4): 335-44.
Ma I & Allan AL. (2011) Stem Cell Rev & Rep 7(2): 292-306.
Hematopoietic Malignancies
Boucher K et al. (2012) . Clin Cancer Res 18(22): 6155-68.
Gerber JM et al. (2012) . Blood 119(15): 3571-77.
Hanke M et al. (2014) . Biomaterials 35(5): 1411-9.
de Leeuw DC et al. (2014) . Cancer Res 74(7): 2094-105.
Man CH et al. (2012) . Blood 119(22): 5133-43.
Nakamura S et al. (2010) . Carcinogenesis 31(11): 2012-21.
Yang Y et al. (2013) . Blood 122(8): 1437-47.
Gerber JM et al. (2012) . Blood 119(15): 3571-77.
Hanke M et al. (2014) . Biomaterials 35(5): 1411-9.
de Leeuw DC et al. (2014) . Cancer Res 74(7): 2094-105.
Man CH et al. (2012) . Blood 119(22): 5133-43.
Nakamura S et al. (2010) . Carcinogenesis 31(11): 2012-21.
Yang Y et al. (2013) . Blood 122(8): 1437-47.
Brain Cancer Cells
Choi SA et al. (2013) . Eur J Cancer 50(1): 137-49.
Rasper M et al. (2010) . Neuro Oncol 12(10): 1024-33.
Sun P et al. (2009) . Stem Cells 27(7): 1473-86.
Rasper M et al. (2010) . Neuro Oncol 12(10): 1024-33.
Sun P et al. (2009) . Stem Cells 27(7): 1473-86.
Breast Cancer Cells
Alam M et al. (2013) . J Biol Chem 288(43): 30892-903.
Atkinson RL et al. (2013) . Breast Cancer Res 15(5): R77.
Azzam DJ et al. (2013) . EMBO Mol Med 5(10): 1502-22.
Buckley NE et al. (2013) . Nucl Acids Res 41(18): 8601-14.
Buijs JT et al. (2012) . Oncogene 31(17): 2164-74.
Chen D et al. (2013). Cancer Res 73(18): 5821-33.
Conti L et al. (2013) . FASEB J 27(12): 4731-44.
Ithimakin S et al. (2013) . Cancer Res 73(5): 1635-46.
Kundu N et al. (2014). Breast Cancer Res TR 143(1): 19-31.
Liu S et al. (2008) . PNAS 105(5): 1680-85.
Liu P et al. (2013) . Brit J Cancer 109(7): 1876-85.
Londoño-Joshi AI et al. (2014) . Mol Cancer Ther 13(4):800-11.
McClements L et al. (2013) Clin Cancer Res 19(14): 3881-93.
Piva M et al. (2014) . EMBO Mol Med 6(1): 66-79.
Rustighi A et al. (2014) . EMBO Mol Med 6(1): 99-119.
Salvador MA et al. (2013) . Clin Cancer Res 19(23): 6520-31.
Vazquez-Martin A et al. (2013) . Cell Cycle 12(22): 3471-77.
Wang X et al. (2013) . Oncogene 32(49): 5512-21.
Yamamoto M et al. (2013) . Nat Commun 4: 2299.
Yu F et al. (2011) . Oncogene 30(18): 2161-272.
Zhou Y et al. (2014) . Cell Death Differ 21(3): 462-74.
Atkinson RL et al. (2013) . Breast Cancer Res 15(5): R77.
Azzam DJ et al. (2013) . EMBO Mol Med 5(10): 1502-22.
Buckley NE et al. (2013) . Nucl Acids Res 41(18): 8601-14.
Buijs JT et al. (2012) . Oncogene 31(17): 2164-74.
Chen D et al. (2013). Cancer Res 73(18): 5821-33.
Conti L et al. (2013) . FASEB J 27(12): 4731-44.
Ithimakin S et al. (2013) . Cancer Res 73(5): 1635-46.
Kundu N et al. (2014). Breast Cancer Res TR 143(1): 19-31.
Liu S et al. (2008) . PNAS 105(5): 1680-85.
Liu P et al. (2013) . Brit J Cancer 109(7): 1876-85.
Londoño-Joshi AI et al. (2014) . Mol Cancer Ther 13(4):800-11.
McClements L et al. (2013) Clin Cancer Res 19(14): 3881-93.
Piva M et al. (2014) . EMBO Mol Med 6(1): 66-79.
Rustighi A et al. (2014) . EMBO Mol Med 6(1): 99-119.
Salvador MA et al. (2013) . Clin Cancer Res 19(23): 6520-31.
Vazquez-Martin A et al. (2013) . Cell Cycle 12(22): 3471-77.
Wang X et al. (2013) . Oncogene 32(49): 5512-21.
Yamamoto M et al. (2013) . Nat Commun 4: 2299.
Yu F et al. (2011) . Oncogene 30(18): 2161-272.
Zhou Y et al. (2014) . Cell Death Differ 21(3): 462-74.
Colon Cancer Cells
Lotti F et al. (2013) . J Exp Med 210(13): 2851-72.
Lu J et al. (2013) . Cancer Cell 23(2): 171-85.
Morris KT et al. (2014) . Brit J Cancer 110: 1211-20.
Ni C et al. (2013) . Cancer Lett 336(1): 174-84.
Swindall AF et al. (2013) . Cancer Res 73(7): 2368-78.
Todaro M et al. (2010) . Gastroenterology 138(6): 2151-62.
Volonté A et al. (2014) . J Immunol 192(1): 523- 32.
Lu J et al. (2013) . Cancer Cell 23(2): 171-85.
Morris KT et al. (2014) . Brit J Cancer 110: 1211-20.
Ni C et al. (2013) . Cancer Lett 336(1): 174-84.
Swindall AF et al. (2013) . Cancer Res 73(7): 2368-78.
Todaro M et al. (2010) . Gastroenterology 138(6): 2151-62.
Volonté A et al. (2014) . J Immunol 192(1): 523- 32.
Head and Neck Cancer Cells
Bertrand G et al. (2014) . Stem Cell Rev Rep 10(1): 114-26.
Clay MR et al. (2010) . Head Neck 32(9): 1195-201.
Krishnamurthy S et al. (2010) . Cancer Res 70(23): 9969-78.
Lo JF et al. (2011) . Cancer Res 71(5): 1912-23.
Xie X et al. (2014) . Oncogene 33(8): 1037-46.
Yu CC et al. (2013) . Cancer Res 73(11): 3425-40.
Zhang M et al. (2014) . Cancer 120(7): 992-1001.
Clay MR et al. (2010) . Head Neck 32(9): 1195-201.
Krishnamurthy S et al. (2010) . Cancer Res 70(23): 9969-78.
Lo JF et al. (2011) . Cancer Res 71(5): 1912-23.
Xie X et al. (2014) . Oncogene 33(8): 1037-46.
Yu CC et al. (2013) . Cancer Res 73(11): 3425-40.
Zhang M et al. (2014) . Cancer 120(7): 992-1001.
Lung Cancer Cells
Bleau AM et al. (2014) . Int J Cancer 135(11):2516-27.
Noto A et al. (2013) . Cell Death Dis 4(12): e947.
Ricci A et al. (2013) . Cell Cycle 12(11): 1696-703.
Sullivan JP et al. (2010) . Cancer Res 70(23): 9937-48.
Yae T et al. (2012) . Nature Comm 3: 883.
Noto A et al. (2013) . Cell Death Dis 4(12): e947.
Ricci A et al. (2013) . Cell Cycle 12(11): 1696-703.
Sullivan JP et al. (2010) . Cancer Res 70(23): 9937-48.
Yae T et al. (2012) . Nature Comm 3: 883.
Ovarian Cancer Cells
Abelson S et al. (2012) . Stem Cells 30(3): 415-24.
Bareiss PM et al. (2013) . Cancer Res 73(17): 5544- 55.
Flesken-Nikitin A et al. (2013) . Nature 495(7440): 241-5.
Kryczek I et al. (2011) . Int J Cancer 130(1): 29-39.
Silva IA et al. (2011) . Cancer Res 71(11): 3991-4001.
Bareiss PM et al. (2013) . Cancer Res 73(17): 5544- 55.
Flesken-Nikitin A et al. (2013) . Nature 495(7440): 241-5.
Kryczek I et al. (2011) . Int J Cancer 130(1): 29-39.
Silva IA et al. (2011) . Cancer Res 71(11): 3991-4001.
Pancreatic Cancer Cells
Cano CE et al. (2014) . Gut 63(6):984-95.
Herreros-Villanueva M et al. (2013) . Oncogenesis 2(8): e61.
Liu L et al. (2014) . Int J Cancer 134(10): 2489- 503.
Rasheed Z et al. (2010) . J Vis Exp (43): 2169.
Herreros-Villanueva M et al. (2013) . Oncogenesis 2(8): e61.
Liu L et al. (2014) . Int J Cancer 134(10): 2489- 503.
Rasheed Z et al. (2010) . J Vis Exp (43): 2169.
Prostate Cancer Cells
Jeter CR et al. (2011) . Oncogene 30: 3833-45.
Le Magnen C et al. (2013) . Clin Cancer Res 19(19): 5361-71.
van den Hoogen C et al. (2010) . Cancer Res 70(12): 5163-73.
Le Magnen C et al. (2013) . Clin Cancer Res 19(19): 5361-71.
van den Hoogen C et al. (2010) . Cancer Res 70(12): 5163-73.
Sarcomas
Sangiolo D et al. (2014) . Cancer Res 74(1): 119-29.
Wang L et al. (2011) . Int J Cancer 128(2): 294-303.
Wang L et al. (2011) . Int J Cancer 128(2): 294-303.
Skin Cancer Cells
Boonyaratanakornkit JB et al. (2010) J Invest Dermatol 130(12): 2799– 808.
Thyroid Cancer Cells
Todaro M et al. (2010) Cancer Res 70(21): 8874-85.
