·¡±è¾±°ä³Ü±ô³Ùâ„¢ Media for Mammary Epithelial Cell Culture
·¡±è¾±°ä³Ü±ô³Ùâ„¢ Mammary Cell Culture Media
Serum-Free, Defined Media for Mammary Epithelial Cell Culture
·¡±è¾±°ä³Ü±ô³Ùâ„¢ is a line of serum-free, defined culture media that supports mammary epithelial monolayer culture and assays for detecting the presence of mammary precursor cells in mammary colony-forming units (Ma-CFUs) or 3D Matrigel®-based cultures. For researchers interested in culturing mammospheres or tumorspheres derived from breast cancer cell lines, visit our
page to learn about the most published commercially available medium that has been designed and optimized for this purpose.
·¡±è¾±°ä³Ü±ô³Ùâ„¢-C: Culture Human Mammary Epithelial Cells
·¡±è¾±°ä³Ü±ô³Ùâ„¢-C Medium is optimized to support the short term culture of human mammary luminal epithelial and myoepithelial cells. It can also be used for the enzymatic dissociation of human mammary tissue when supplemented with Collagenase/Hyaluronidase. Watch this video to learn more.
·¡±è¾±°ä³Ü±ô³Ùâ„¢-B: Detect Human or Mouse Mammary Colony-Forming Units (Ma-CFUs)
·¡±è¾±°ä³Ü±ô³Ùâ„¢-B has species-specific formulations for the culture and assay of either human or mouse mammary cells. Detect the presence of mammary precursor cells in Ma-CFUs for quantification, or in 3D Matrigel®-based cultures for an environment that is representative of the in vivo mammary duct structure.
Mammary Cell Culture Products
·¡±è¾±°ä³Ü±ô³Ùâ„¢-B Medium (Human)
Recommended for:
Colony-forming unit (CFU) or 3D Matrigel®-based assay for differential assessment of progenitor cell content
Species:
Human
·¡±è¾±°ä³Ü±ô³Ùâ„¢-B Medium (Mouse)
Recommended for:
Colony-forming unit (CFU) or 3D Matrigel®-based assay for differential assessment of progenitor cell content
Species:
Mouse
·¡±è¾±°ä³Ü±ô³Ùâ„¢-C Human Medium Kit
Recommended for:
Short-term culture of primary human mammary epithelial cells
Species:
Human
²Ñ²¹³¾³¾´Ç°ä³Ü±ô³Ùâ„¢ Human Medium Kit
Recommended for:
Culturing mammospheres or tumorspheres
Species:
Human
·¡±è¾±°ä³Ü±ô³Ùâ„¢ Plus Medium
Recommended for:
Culturing of epithelial stem cells (except human keratinocytes or human airway cells)
Species:
Human, mouse, and rat
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Key Applications
Publications on Normal Mammary Cells
EpiCult™-B (Human)
Eirew P et al. (2008) Nat Med 14(12): 1384-9.
Raouf A et al. (2008) Cell Stem Cell 3(1): 109-18.
Stingl J et al. (2001) Breast Cancer Res Treat 67(2): 93-109.
Raouf A et al. (2008) Cell Stem Cell 3(1): 109-18.
Stingl J et al. (2001) Breast Cancer Res Treat 67(2): 93-109.
EpiCult™-B (Mouse)
Yamaji D et al. (2009) Genes & Development 23(20): 2382-7.
Stingl J et al. (2001) Breast Cancer Res Treat 67(2): 93-109.
Stingl J et al. (2001) Breast Cancer Res Treat 67(2): 93-109.
Publications on Cancerous Cells
EpiCult™-B (Human)
Kawakubo T et al. (2013) Carcinogenesis 35(3): 714-26.
D'Uva G et al. (2013) PLoS one 8(11) e80742.
Law JH et al. (2010) PLoS One 5(9): e12661.
Diehn M et al. (2009) Nature 458(7239): 780-3.
Lindvall C et al. (2006) J Biol Chem 281(46): 35081-7.
D'Uva G et al. (2013) PLoS one 8(11) e80742.
Law JH et al. (2010) PLoS One 5(9): e12661.
Diehn M et al. (2009) Nature 458(7239): 780-3.
Lindvall C et al. (2006) J Biol Chem 281(46): 35081-7.
EpiCult™-B (Mouse)
Chen EP et al. (2014) Carcinogenesis 35(8):1788-97.
Teissedre B et al. (2009) PLoS ONE 4(2): e4537.
Shimono Y et al. (2009) Cell 138(3): 592-603.
Shafee N et al. (2008) Cancer Res 68(9): 3243-50.
Teissedre B et al. (2009) PLoS ONE 4(2): e4537.
Shimono Y et al. (2009) Cell 138(3): 592-603.
Shafee N et al. (2008) Cancer Res 68(9): 3243-50.
EpiCult™-C (Human)
Zhang L et al. (2013) Sci Transl Med 5(180): 180ra48.
Publications on Non-Mammary Tissue
EpiCult™-B (Human)
Ghosh M et al. (2013) Am J Respir Cell Mol Biol 49(6): 1127-34.
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