Immune Tolerance
Immune tolerance is critical to prevent the development of autoimmune and inflammatory diseases. Although the concept of tolerance has long been established, researchers continue to uncover previously unknown mechanisms and therapeutic applications of immune tolerance.
Below is a collection of scientific resources for your immune tolerance research.
Featured
Regulatory T Cells
Ethan Shevach and Todd Davidson. This poster provides an updated overview of the development, phenotype and functions of regulatory T cells, in particular those subsets that express the transcription factor forkhead box P3 (FOXP3).
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Andreia Costa, BScAndreia Costa describes her work in the Lund lab investigating the role of regulatory T cells in neurotophic infections -
How to Count Cells Using a HemocytometerHow to prepare and count stained cell samples using a hemocytometer for both total nucleated cell counts and viable cell counts -
Acquiring DNA or RNA from Positively Selected CellsCells positvely selected with the EasySep™ system do not interfere with most downstream applications -
How to Isolate Cells Directly from Whole Blood Using the Easy 50 EasySep™ Magnet: 30-Minute ProtocolHow to isolate highly purified immune cells directly from whole blood samples of up to 30 mL with and the protocol takes as little as 30 minutes from start to finish -
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation TechnologyLearn how to perform the EasySep™ pour-off -
How to Isolate Cells with EasySep™ Column-Free Cell Separation TechnologyIsolation of human CD4+ T cells by negative selection using EasySep™ to separate highly purified cells in as little as 8 minutes -
How to Prepare a Single Cell Suspension from Frozen Samples and Prevent Cell ClumpingLearn how to harvest cells from a frozen starting sample and prepare a single cell suspension prior to performing cell isolation -
How to Deplete Red Blood Cells from Small Volume Samples with HetaSep™How to use an erythrocyte aggregation agent to remove red blood cells (RBCs) from small volume blood samples prior to colony-forming unit (CFU) assays -
Automate Cell Isolation for Up to 16 Samples with the RoboSep™-16 Cell Separation InstrumentFeatures and benefits of using the fully automated cell isolation platform RoboSep™-16 which allows you to efficiently isolate cells from up to 16 samples simultaneously -
Comparing Cell Separation Platforms from ϳԹϳԹ is a leader in cell separation technology, & we offer multiple cell separation platforms, to suit different research needs -
Improving Cell Recovery with EasySep™ Negative Selection KitsTechnical tip from our dedicated team of Product and Scientific Support specialists -
Considerations for Flow Cytometry GatingTo achieve accurate results, it is important to use an optimal gating strategy when assessing cell samples via flow cytometry
