Immune Tolerance
Immune tolerance is critical to prevent the development of autoimmune and inflammatory diseases. Although the concept of tolerance has long been established, researchers continue to uncover previously unknown mechanisms and therapeutic applications of immune tolerance.
Below is a collection of scientific resources for your immune tolerance research.
Featured
Regulatory T Cells
Ethan Shevach and Todd Davidson. This poster provides an updated overview of the development, phenotype and functions of regulatory T cells, in particular those subsets that express the transcription factor forkhead box P3 (FOXP3).
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How to Prepare a Single Cell Suspension from Frozen Samples and Prevent Cell ClumpingLearn how to harvest cells from a frozen starting sample and prepare a single cell suspension prior to performing cell isolation -
How to Count Cells Using a HemocytometerHow to prepare and count stained cell samples using a hemocytometer for both total nucleated cell counts and viable cell counts -
Assessment of Your Starting Sample and Calculation of Recovery from Cell Isolation ProceduresSetting aside a small portion of your starting sample allows you to assess the frequency of desired cells in the sample prior to isolation -
Before Starting a Run on RoboSep™-SBasic overview on how to use and maintain your RoboSep™-S; steps to follow before starting a cell separation run -
Isolate Multiple Cell Types from a Single Sample with Sequential IsolationSequential separation, for higher recovery of purified cells from limited sample size and isolation of multiple cell types, is ideal for chimerism analysis -
How to Isolate Cells Directly from Whole Blood - EasySep™ Direct Protocol, EasyEights MagnetHow to isolate highly purified immune cells from up to 16 whole blood samples at once with EasySep™ Direct on the EasyEights EasySep™ Magnet and the protocol takes as little as 25 minutes from start to finish -
How SepMate™ PBMC Isolation Tubes Work: From Whole Blood to PBMCs in 15 MinutesSepMate™, an innovative tube that enables consistent and hassle-free peripheral blood mononuclear cell (PBMC) isolation, for fast and easy PBMC isolation from whole blood in just 15 minutes using density gradient centrifugation -
Loading the RoboSep™-S Carousel and Starting a RunBasic overview on how to use and maintain your RoboSep™-S; steps to load the instrument with samples and reagents and start a cell separation run -
How to Isolate Cells with EasySep™ Column-Free Cell Separation TechnologyIsolation of human CD4+ T cells by negative selection using EasySep™ to separate highly purified cells in as little as 8 minutes -
Processing Multiple Samples at One Time with the EasyEights™ MagnetThe EasyEights™ EasySep™ Magnet is for cell separation procedures using selected EasySep™ reagents when simultaneously processing multiple samples -
Using Cell Density Gradients to Optimize Your Cell IsolationsRosetteSep™, a unique immunodensity cell isolation platform, isolates highly purified cells by density gradient centrifugation without columns or magnets -
Isolating Cells from Atypical SamplesTechnical tip from our dedicated team of Product and Scientific Support specialists
