Intestinal Organoids
The intestinal epithelium is a rapidly renewing tissue that can undergo complete cellular replacement every four to five days. These regenerative properties make the intestinal epithelium an attractive system for researchers studying epithelial regeneration, adult stem cell biology, disease modelling and cancer biology, both within and outside the context of the intestine.
Explore the resources below for information to support your intestinal research and the growth of intestinal organoids.
Featured
E-Book: Proven Protocols for Intestinal Organoid Culture
From getting started with intestinal organoids to advanced assays, this e-book provides curated protocols for the isolation and culture of human-derived intestinal organoids. Find proven protocols for a wide variety of well-established assays from immunocytochemical staining, toxicity testing, genetic manipulation, and monolayer differentiation.
Download Now >Key Applications of Intestinal Organoids
Crypt-Derived Intestinal Organoids
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Sato T et al. (2011) Long-term expansion of epithelial organoids from human colon, adenoma, adenocarcinoma, and Barrett’s epithelium. Gastroenterology 141(5): 1762–72.
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Ootani A et al. (2009) Sustained in vitro intestinal epithelial culture within a Wnt-dependent stem cell niche. Nat Med 15(6): 701–6.
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Sato T et al. (2011) Long-term expansion of epithelial organoids from human colon, adenoma, adenocarcinoma, and Barrett’s epithelium. Gastroenterology 141(5): 1762–72.
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PSC-Derived Intestinal Organoids
Spence JR et al. (2011) Directed differentiation of human pluripotent stem cells into intestinal tissue in vitro. Nature 470(7332): 105–9.
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Múnera JO et al. (2017) Methods Mol Biol 1597: 167–177.
Intestinal Cell Biology
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Dotti I et al. (2016) Alterations in the epithelial stem cell compartment could contribute to permanent changes in the mucosa of patients with ulcerative colitis. Gut. Epub ahead of print, DOI: 10.1136/gutjnl-2016-312609.
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Oudhoff MJ et al. (2016) SETD7 controls intestinal regeneration and tumorigenesis by regulating Wnt/β-catenin and Hippo/YAP signaling. Dev Cell 37(1): 47–57.
Sasaki N et al. (2016) Reg4+ deep crypt secretory cells function as epithelial niche for Lgr5+ stem cells in colon. Proc Natl Acad Sci U S A 113(37): E5399-407.
Schewe M et al. (2016) Secreted phospholipases A2 are intestinal stem cell niche factors with distinct roles in homeostasis, inflammation, and cancer. Cell Stem Cell 19(1): 38–51.
Workman MJ et al. (2017) Nat Med 23(1): 49–59.
Zietek T et al. (2015) Intestinal organoids for assessing nutrient transport, sensing and incretin secretion. Sci Rep 5: 16831.
Mohanan V et al. (2018) Science 359(6380): 1161–1166.
Wang B et al. (2018) Cell Stem Cell 22(2): 206–220.
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Rong S et al. (2017) J Lipid Res 58(10): 1988–1998.
Morgenstern Y et al. (2017) Embo J 36(20): 3046–3061.
Kraft CL et al. (2017) Oncotarget 8(61): 102923–102933.
Dotti I et al. (2016) Alterations in the epithelial stem cell compartment could contribute to permanent changes in the mucosa of patients with ulcerative colitis. Gut. Epub ahead of print, DOI: 10.1136/gutjnl-2016-312609.
Igarashi M & Guarente L. (2016) mTORC1 and SIRT1 cooperate to foster expansion of gut adult stem cells during calorie restriction. Cell 166(2): 436–50.
Oudhoff MJ et al. (2016) SETD7 controls intestinal regeneration and tumorigenesis by regulating Wnt/β-catenin and Hippo/YAP signaling. Dev Cell 37(1): 47–57.
Sasaki N et al. (2016) Reg4+ deep crypt secretory cells function as epithelial niche for Lgr5+ stem cells in colon. Proc Natl Acad Sci U S A 113(37): E5399-407.
Schewe M et al. (2016) Secreted phospholipases A2 are intestinal stem cell niche factors with distinct roles in homeostasis, inflammation, and cancer. Cell Stem Cell 19(1): 38–51.
Workman MJ et al. (2017) Nat Med 23(1): 49–59.
Zietek T et al. (2015) Intestinal organoids for assessing nutrient transport, sensing and incretin secretion. Sci Rep 5: 16831.
Mohanan V et al. (2018) Science 359(6380): 1161–1166.
Wang B et al. (2018) Cell Stem Cell 22(2): 206–220.
Wichert R et al. (2017) Cell Rep 21(8): 2090–2103.
Haber AL et al. (2017) Nature 551(7680): 333–339.
Kraiczy J et al. (2017) Gut.
Zhou W et al. (2018) J Cell Physiol 233(4): 3465–3475.
Hahn S et al. (2017) Sci Rep 7(1): 2435.
Rong S et al. (2017) J Lipid Res 58(10): 1988–1998.
Morgenstern Y et al. (2017) Embo J 36(20): 3046–3061.
Kraft CL et al. (2017) Oncotarget 8(61): 102923–102933.
Epithelial Cell Biology
Blokzijl F et al. (2016) Tissue-specific mutation accumulation in human adult stem cells during life. Nature 538(7624): 260–4.
Kikuchi I et al. (2016) Nat Commun 7: 12887.
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Saxena A et al. (2017) Am J Physiol Gastrointest Liver Physiol 313(1): G26–G38.
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Nakajo A et al. (2016) J Cell Biol 212(3): 297–306.
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Cancer Research
Borovski T et al. (2017) Gut 66(6): 1106–1115.
Fernández-Majada V et al. (2016) Nat Commun 7: 12508.
Fujii M et al. (2016) Cell Stem Cell 18(6): 827–38.
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Duhachek-Muggy S et al. (2017) Radiat Res 188(5): 469–475.
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Regan JL et al. (2017) Cell Rep 21(10): 2813–2828.
Novellasdemunt L et al. (2017) Cell Rep 21(3): 612–627.
Banerjee A et al. (2016) Oncotarget 7(27): 41432–41444.
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Fernández-Majada V et al. (2016) Nat Commun 7: 12508.
Fujii M et al. (2016) Cell Stem Cell 18(6): 827–38.
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Yan HHN et al. (2017) Gut 66(9): 1645–1656.
Yum MK et al. (2016) Cancer Res 76(15): 4559–68.
Duhachek-Muggy S et al. (2017) Radiat Res 188(5): 469–475.
Karki R et al. (2016) Nature 540: 583–587.
Dame MK et al. (2018) Development 145(6).
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Roper J et al. (2018) Nat Protoc 13(2): 217–234.
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Regan JL et al. (2017) Cell Rep 21(10): 2813–2828.
Novellasdemunt L et al. (2017) Cell Rep 21(3): 612–627.
Banerjee A et al. (2016) Oncotarget 7(27): 41432–41444.
Liu W et al. (2016) Oncogene 35(40): 5237–5247.
Precision Medicine Screening
Vlachogiannis G et al. (2018) Science 359(6378): 920–926.
Beekman JM. (2016) Pediatr Pulmonol 51(S44): S23–S34.
Hagemeijer MC et al. (2018) J Cyst Fibros 17(2s): S46–S51.
Beekman JM. (2016) Pediatr Pulmonol 51(S44): S23–S34.
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Therapeutic Development
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Disease Modeling
Dekkers JF et al. (2016) Eur Respir J 48(2): 451–8.
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Dilly AK et al. (2017) Oncotarget 8(63): 106888–106900.
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Huang K et al. (2018) Pediatr Res 83(5): 1031–1040.
Suzuki K et al. (2018) J Gastroenterol.
Dilly AK et al. (2017) Oncotarget 8(63): 106888–106900.
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Donaldson DS et al. (2016) PLOS Pathog 12(12): E1006075.
Pattison AM et al. (2016) Infect Immun 84(10): 3083–91.
Intestinal Immunity and Inflammation
Gerbe F et al. (2016) Nature 529(7585): 226–30.
Ibiza S et al. (2016) Nature 535(7612): 440–443.
Lindemans CA et al. (2015) Nature 528(7583): 560–564.
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Sharma D et al. (2018) Gastroenterology 154(4): 948–964.
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Oancea I et al. (2017) Gut 66(1): 59–69.
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Wang Y et al. (2017) Mucosal Immunol 10(2): 373–384.
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Microbiomics and Microbial Pathogenesis
Bartfeld S. (2016) Dev Biol 420(2): 262–270.
In Jg et al. (2016) Nat Rev Gastroenterol Hepatol 13(11): 633–642.
Tao L et al. (2016) Nature 538(7625): 350–355.
Wang J et al. (2018) Gastroenterology 154(6): 1737–1750.
Leber A et al. (2018) Front Immunol 9: 363.
Burger E et al. (2018) Cell Host Microbe 23(2): 177–190.
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Yahiro K et al. (2018) Cell Death Discov 4: 22.
Entwistle LJ et al. (2017) Cell Host Microbe 22(4): 484–493.
Dhawan S et al. (2016) Am J Physiol Gastrointest Liver Physiol 311(5): G920–G933.
Wang Y et al. (2017) Science 357(6354): 912–916.
In Jg et al. (2016) Nat Rev Gastroenterol Hepatol 13(11): 633–642.
Tao L et al. (2016) Nature 538(7625): 350–355.
Wang J et al. (2018) Gastroenterology 154(6): 1737–1750.
Leber A et al. (2018) Front Immunol 9: 363.
Burger E et al. (2018) Cell Host Microbe 23(2): 177–190.
Zhang D et al. (2017) Sci Rep 7(1): 12621.
Yahiro K et al. (2018) Cell Death Discov 4: 22.
Entwistle LJ et al. (2017) Cell Host Microbe 22(4): 484–493.
Dhawan S et al. (2016) Am J Physiol Gastrointest Liver Physiol 311(5): G920–G933.
Wang Y et al. (2017) Science 357(6354): 912–916.
