²Ñ²¹³¾³¾´Ç°ä³Ü±ô³Ùâ„¢ Media for Mammosphere or Tumorsphere Culture
²Ñ²¹³¾³¾´Ç°ä³Ü±ô³Ùâ„¢ Media for Mammary Cell Culture
Defined, Serum-Free Medium for Mammosphere and Tumorsphere Culture
²Ñ²¹³¾³¾´Ç°ä³Ü±ô³Ùâ„¢ is the most published commercially available medium for the culture of mammospheres and tumorspheres. Mammospheres are cultured from primary breast tissue or cell lines, while tumorspheres are cultured from primary tumor cells and cancer cell lines from multiple tissues. ²Ñ²¹³¾³¾´Ç°ä³Ü±ô³Ùâ„¢ has been used with a variety of breast cancer cell lines for tumorsphere generation, including MCF7, MCF10A, SKBR3, MDA-MB-231, AU565, SUM149 and BT474. While leading breast cancer researchers use ²Ñ²¹³¾³¾´Ç°ä³Ü±ô³Ùâ„¢ for their research, this defined and serum-free medium has also been used in sphere assays with cells derived from ovaries, lung, colon and other tissues.
Culture Mammospheres and Tumorspheres
Three-dimensional sphere cultures provide higher physiological relevance than traditional monolayer cultures. The mammosphere culture system involves seeding mammary epithelial cells at low density in an environment that prevents their adherence to a substratum and enables their proliferation in suspension as spherical clusters. This system is capable of propagating mammary stem and progenitor cells in vitro and supports tumorsphere culture of breast cancer stem cells and cell lines. Watch this video to learn how you can easily generate mammospheres and tumorspheres using ²Ñ²¹³¾³¾´Ç°ä³Ü±ô³Ùâ„¢.
Breast Cancer Research
Leading breast cancer researchers choose MammoCult™ to culture tumorspheres derived from breast cancer stem cells or cell lines. In addition to using MammoCult™, advance your research with our full suite of cancer research tools including specialized cell culture media, cell isolation tools and stem cell detection kits. Watch this video to see how and are also helping researchers in the fight against breast cancer.
Why Use ²Ñ²¹³¾³¾´Ç°ä³Ü±ô³Ùâ„¢?
- Serum-free, defined formulation.
- Generates a large number of passagable mammospheres and tumorspheres.
- Supports long-term maintenance of breast cancer cell lines.
- The most published commercially available medium for sphere culture from mammary tissue.
Mammary Cell Culture Products
²Ñ²¹³¾³¾´Ç°ä³Ü±ô³Ùâ„¢ Medium (Human)
Recommended for:
Culturing mammospheres or tumorspheres
Species:
Human
·¡±è¾±°ä³Ü±ô³Ùâ„¢-B Medium (Human)
Recommended for:
Colony-forming unit (CFU) assay for differential assessment of progenitor cell content
Species:
Human
·¡±è¾±°ä³Ü±ô³Ùâ„¢-B Medium (Mouse)
Recommended for:
Colony-forming unit (CFU) assay for differential assessment of progenitor cell content
Species:
Mouse
·¡±è¾±°ä³Ü±ô³Ùâ„¢-C Human Medium Kit
Recommended for:
Short-term culture of primary human mammary epithelial cells
Species:
Human
·¡±è¾±°ä³Ü±ô³Ùâ„¢ Plus Medium
Recommended for:
Culturing of human, mouse, and rat epithelial stem cells (except human keratinocytes or human airway cells)
Species:
Human, mouse, and rat
Collapse Details
Scientific Resources
Wallchart
SnapShot: Breast Cancer
Brochure
Tools for Cancer Research
Video
How to Count 3D Sphere Cultures (Mammospheres, Neurospheres, Cancer Spheroids, and PSC Aggregates)
Mini Review
Mammary Stem Cells
Key Applications
Normal Breast Cell Lines
MCF10A
Kendellen MF et al. (2014) Oncogene 33(10): 1297-305.
MCF10A-SNAl1, MCF10A-SNAl1-R
Lim S et al. (2013) PLoS One 8(6): e66558.
HMLER
Burke AR et al. (2012) Biomaterials 33(10): 2961-70.
Qiao Y et al. (2011) Cancer Res 71(8):3076-86.
Kendellen MF et al. (2014) Oncogene 33(10): 1297-305.
MCF10A-SNAl1, MCF10A-SNAl1-R
Lim S et al. (2013) PLoS One 8(6): e66558.
HMLER
Burke AR et al. (2012) Biomaterials 33(10): 2961-70.
Qiao Y et al. (2011) Cancer Res 71(8):3076-86.
Breast Cancer Cell Lines
HBL-100
Smart CE et al. (2013) PLoS One 8(6): e64388.
KPL-1
Smart CE et al. (2013) PLoS One 8(6): e64388.
MDA-MB-453
Zhao S et al. (2013) 330(1): 41-8.
SUM 159
Kendellen MF et al. (2014) Oncogene 33(10): 1297-305.
Smart CE et al. (2013) PLoS One 8(6): e64388.
Del Vecchio CA et al. (2012) Cancer Res 72(10): 2657-71.
Korkaya H et al. (2012) 47(4): 570-84.
BT-20
Smart CE et al. (2013) PLoS One 8(6): e64388.
HCC202
Deng et al. (2010) PLoS One 5(4): e10277.
MCF7
D'Assoro AB et al. (2014) Oncogene 33(5): 599-610.
Kundu N et al. (2014) 143(1): 19-31.
Rajabi H et al. (2014) Oncogene 33(13): 1680-9.
Alam M et al. (2013) J Biol Chem 288(43): 30892-903.
Bhat-Nakshatri P et al. (2013) Sci Rep 3: 2530.
Bianco C et al. (2013) J Cell Physiol 228(6): 1174-88.
Cho JH et al. (2013) J Biol Chem 288(5): 3406-18.
Lee JH et al. (2013) Oncol Rep 29(3): 917-24.
Smart CE et al. (2013) PLoS One 8(6): e64388.
Vares G et al. (2013) PLoS One 8(10): e77124.
Wei X et al. (2013) 24(4): 658-68.
Wolf J et al. (2013) 15(6): R109.
Zhao S et al. (2013) Cancer Lett 330(1): 41-8.
Gilani RA et al. (2012) Breast Cancer Res Treat 135(3): 681-92.
Korkaya H et al. (2012) Mol Cell (47(4): 570-84.
Palaniyandi K et al. (2012) 4(7): 214-222.
Wong NK et al. (2012) Cancer Med 1(2): 105-13.
Wu F et al. (2012) Cell Signal 24(11): 1989-98.
Zhao S et al. (2012) Cancer Lett 317(2): 192-8.
Kumar A et al. (2011) PLoS One 6(6): e20701.
Deng et al. (2010) PLoS One 5(4): e10277.
MDA-MB-468
Alam M et al. (2013) J Biol Chem 288(43): 30892-903.
T-47D
Axlund SD et al. (2013) Horm Cancer 4(1): 36-49.
Smart CE et al. (2013) PLoS One 8(6): e64388.
Vares G et al. (2013) PLoS One 8(10): e77124.
Deng et al. (2010) PLoS One 5(4): e10277.
BT-474
D'Assoro AB et al. (2014) Oncogene 33(5): 599-610.
Smart CE et al. (2013) PLoS One 8(6): e64388.
HCC1599
Zhang CC et al. (2012) Clin Cancer Res 18(18): 5008-19.
MDA-MB-231
D'Assoro AB et al. (2014) Oncogene 33(5): 599-610.
Kendellen MF et al. (2014) Oncogene 33(10): 1297-305.
Kundu N et al. (2014) Breast Cancer Res 143(1): 19-31.
Rajabi H et al. (2014) Oncogene 33(13): 1680-9.
Bhat-Nakshatri P et al. (2013) Sci Rep 3: 2530.
Dai L et al. (2013) Am J Pathol 182(2): 577-85.
Di Cello F et al. (2013) Biochem Biophys Res Commun 434(1): 70-4.
Han YK et al. (2013) 430(4): 1329-33.
Lee JH et al. (2013) Oncol Rep 29(3): 917-24.
Lim S et al. (2013) PLoS One 8(6): e66558.
Shen J et al. (2013) ACS Nano 7(11): 9867-80.
Wolf J et al. (2013) 15(6): R109.
Hu K et al. (2012) Breast Cancer Res 14(1): R22.
Rao R et al. (2012) Mol Cancer Ther 11(4): 973-83.
Stratford AL et al. (2012) Stem Cells 30(7): 1338-48.
SKBR3
Kundu N et al. (2014) Breast Cancer Res 143(1): 19-31.
Alam M et al. (2013) J Biol Chem 288(43): 30892-903.
Smart CE et al. (2013) PLoS One 8(6): e64388.
TMD-231
Bhat-Nakshatri P et al. (2013) Sci Rep 3: 2530.
GI-101A
Wang H et al. (2012) J Transl Med 10(1): 167.
Hs578T
Smart CE et al. (2013) PLoS One 8(6): e64388.
MDA-MB-436
Bhat-Nakshatri P et al. (2013) Sci Rep 3: 2530.
Smart CE et al. (2013) PLoS One 8(6): e64388.
SUM149
D'Assoro AB et al. (2014) Oncogene 33(5): 599-610.
Kendellen MF et al. (2014) Oncogene 33(10): 1297-305.
Qiu M et al. (2013) Cancer Lett 328(2): 261-70.
Wolf J et al. (2013) 15(6): R109.
Del Vecchio CA et al. (2012) Cancer Res 72(10): 2657-71.
Hu K et al. (2012) Breast Cancer Res 14(1): R22.
Stratford AL et al. (2012) Stem Cells 30(7): 1338-48.
Zhang CC et al. (2012) Clin Cancer Res 18(18): 5008-19.
ZR-75-1
Wu F et al. (2012) Cell Signal 24(11): 1989-98.
Deng et al. (2010) PLoS One 5(4): e10277.
Smart CE et al. (2013) PLoS One 8(6): e64388.
KPL-1
Smart CE et al. (2013) PLoS One 8(6): e64388.
MDA-MB-453
Zhao S et al. (2013) 330(1): 41-8.
SUM 159
Kendellen MF et al. (2014) Oncogene 33(10): 1297-305.
Smart CE et al. (2013) PLoS One 8(6): e64388.
Del Vecchio CA et al. (2012) Cancer Res 72(10): 2657-71.
Korkaya H et al. (2012) 47(4): 570-84.
BT-20
Smart CE et al. (2013) PLoS One 8(6): e64388.
HCC202
Deng et al. (2010) PLoS One 5(4): e10277.
MCF7
D'Assoro AB et al. (2014) Oncogene 33(5): 599-610.
Kundu N et al. (2014) 143(1): 19-31.
Rajabi H et al. (2014) Oncogene 33(13): 1680-9.
Alam M et al. (2013) J Biol Chem 288(43): 30892-903.
Bhat-Nakshatri P et al. (2013) Sci Rep 3: 2530.
Bianco C et al. (2013) J Cell Physiol 228(6): 1174-88.
Cho JH et al. (2013) J Biol Chem 288(5): 3406-18.
Lee JH et al. (2013) Oncol Rep 29(3): 917-24.
Smart CE et al. (2013) PLoS One 8(6): e64388.
Vares G et al. (2013) PLoS One 8(10): e77124.
Wei X et al. (2013) 24(4): 658-68.
Wolf J et al. (2013) 15(6): R109.
Zhao S et al. (2013) Cancer Lett 330(1): 41-8.
Gilani RA et al. (2012) Breast Cancer Res Treat 135(3): 681-92.
Korkaya H et al. (2012) Mol Cell (47(4): 570-84.
Palaniyandi K et al. (2012) 4(7): 214-222.
Wong NK et al. (2012) Cancer Med 1(2): 105-13.
Wu F et al. (2012) Cell Signal 24(11): 1989-98.
Zhao S et al. (2012) Cancer Lett 317(2): 192-8.
Kumar A et al. (2011) PLoS One 6(6): e20701.
Deng et al. (2010) PLoS One 5(4): e10277.
MDA-MB-468
Alam M et al. (2013) J Biol Chem 288(43): 30892-903.
T-47D
Axlund SD et al. (2013) Horm Cancer 4(1): 36-49.
Smart CE et al. (2013) PLoS One 8(6): e64388.
Vares G et al. (2013) PLoS One 8(10): e77124.
Deng et al. (2010) PLoS One 5(4): e10277.
BT-474
D'Assoro AB et al. (2014) Oncogene 33(5): 599-610.
Smart CE et al. (2013) PLoS One 8(6): e64388.
HCC1599
Zhang CC et al. (2012) Clin Cancer Res 18(18): 5008-19.
MDA-MB-231
D'Assoro AB et al. (2014) Oncogene 33(5): 599-610.
Kendellen MF et al. (2014) Oncogene 33(10): 1297-305.
Kundu N et al. (2014) Breast Cancer Res 143(1): 19-31.
Rajabi H et al. (2014) Oncogene 33(13): 1680-9.
Bhat-Nakshatri P et al. (2013) Sci Rep 3: 2530.
Dai L et al. (2013) Am J Pathol 182(2): 577-85.
Di Cello F et al. (2013) Biochem Biophys Res Commun 434(1): 70-4.
Han YK et al. (2013) 430(4): 1329-33.
Lee JH et al. (2013) Oncol Rep 29(3): 917-24.
Lim S et al. (2013) PLoS One 8(6): e66558.
Shen J et al. (2013) ACS Nano 7(11): 9867-80.
Wolf J et al. (2013) 15(6): R109.
Hu K et al. (2012) Breast Cancer Res 14(1): R22.
Rao R et al. (2012) Mol Cancer Ther 11(4): 973-83.
Stratford AL et al. (2012) Stem Cells 30(7): 1338-48.
SKBR3
Kundu N et al. (2014) Breast Cancer Res 143(1): 19-31.
Alam M et al. (2013) J Biol Chem 288(43): 30892-903.
Smart CE et al. (2013) PLoS One 8(6): e64388.
TMD-231
Bhat-Nakshatri P et al. (2013) Sci Rep 3: 2530.
GI-101A
Wang H et al. (2012) J Transl Med 10(1): 167.
Hs578T
Smart CE et al. (2013) PLoS One 8(6): e64388.
MDA-MB-436
Bhat-Nakshatri P et al. (2013) Sci Rep 3: 2530.
Smart CE et al. (2013) PLoS One 8(6): e64388.
SUM149
D'Assoro AB et al. (2014) Oncogene 33(5): 599-610.
Kendellen MF et al. (2014) Oncogene 33(10): 1297-305.
Qiu M et al. (2013) Cancer Lett 328(2): 261-70.
Wolf J et al. (2013) 15(6): R109.
Del Vecchio CA et al. (2012) Cancer Res 72(10): 2657-71.
Hu K et al. (2012) Breast Cancer Res 14(1): R22.
Stratford AL et al. (2012) Stem Cells 30(7): 1338-48.
Zhang CC et al. (2012) Clin Cancer Res 18(18): 5008-19.
ZR-75-1
Wu F et al. (2012) Cell Signal 24(11): 1989-98.
Deng et al. (2010) PLoS One 5(4): e10277.
Other Tissues
Colon
KWu G et al. (2013) Cancer Biother Radiopharm 28(4): 298-302.
Huang X et al. (2012) Surg Oncol 21(2): 103-7.
Lung
Hassan KA er al. (2013) Clin Cancer Res 19(8): 1972-80.
Nerve Sheath
Dai L et al. (2013) Am J Pathol 182(2): 577-85.
Ovaries
Dai L et al. (2013) Am J Pathol 182(2): 577-85.
Meng F et al. (2013) Br J Cancer 108(3): 579-86.
Meng F et al. (2013) . Int J Oncol 43(2): 495-502.
Pease JC et al. (2012) . Biol Open 1(7): 622-8.
Xu CX et al. (2012) J Biol Chem 287(42): 34970-8.
KWu G et al. (2013) Cancer Biother Radiopharm 28(4): 298-302.
Huang X et al. (2012) Surg Oncol 21(2): 103-7.
Lung
Hassan KA er al. (2013) Clin Cancer Res 19(8): 1972-80.
Nerve Sheath
Dai L et al. (2013) Am J Pathol 182(2): 577-85.
Ovaries
Dai L et al. (2013) Am J Pathol 182(2): 577-85.
Meng F et al. (2013) Br J Cancer 108(3): 579-86.
Meng F et al. (2013) . Int J Oncol 43(2): 495-502.
Pease JC et al. (2012) . Biol Open 1(7): 622-8.
Xu CX et al. (2012) J Biol Chem 287(42): 34970-8.
